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| DOI: | 10.1093/aob/mcn016 |
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| Web URL(s): | https://academic.oup.com/aob/article/101/6/845/184667/Development-of-a-Genomic-Microsatellite-Library-in
Last checked: 03/02/2017
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | King, J.;
Thorogood, D.;
Edwards, K. J.;
Armstead, I. P.;
Roberts, L.;
Skøt, K.;
Hanley, Z.;
King, I. P. |
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| Author Affiliation: | King, J., Thorogood, Roberts, Skøt, and King, I. P.: Institute of Grassland and Environmental Research, Plus Gogerddan, Aberystwyth, United Kingdom; Edwards: School of Biological Sciences, University of Bristol, Bristol, United Kingdom; Hanley: Vialactia Biosciences, Auckland, New Zealand |
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| Title: | Development of a genomic microsatellite library in perennial ryegrass (Lolium perenne) and its use in trait mapping |
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| Source: | Annals of Botany. Vol. 101, No. 6, April 2008, p. 845-853. |
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| Publishing Information: | London, Oxford University Press |
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| # of Pages: | 9 |
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| Keywords: | TIC Keywords: Microsatellites; Gene mapping; Lolium perenne; Genetic markers; Databases; DNA; Genetics
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| Abstract/Contents: | "Background and Aims: Perennial ryegrass (Lolium perenne) is one of the key forage and amenity grasses throughout the world. In the UK it accounts for 70 % of all agricultural land use with an estimated farm gate value of £6 billion per annum. However, in terms of the genetic resources available, L. perenne has lagged behind other major crops in Poaceae. The aim of this project was therefore the construction of a microsatellite-enriched genomic library for L. perenne to increase the number of genetic markers available for both marker-assisted selection in breeding programmes and gene isolation. Methods: Primers for 229 non-redundant microsatellite markers were designed and used to screen two L. perenne genotypes, one amenity and one forage. Of the 229 microsatellites, 95 were found to show polymorphism between amenity and forage genotypes. A selection of microsatellite primers was selected from these 95 and used to screen two mapping populations derived from intercrossing and backcrossing the two forage and amenity grass genotypes. Key Results and Conclusions: The utility of the resulting genetic maps for analysis of the genetic control of target traits was demonstrated by the mapping of genes associated with heading date to linkage groups 4 and 7." |
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| Language: | English |
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| References: | 37 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
King, J., D. Thorogood, K. J. Edwards, I. P. Armstead, L. Roberts, K. Skøt, et al. 2008. Development of a genomic microsatellite library in perennial ryegrass (Lolium perenne) and its use in trait mapping. Ann. Bot. 101(6):p. 845-853. |
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| DOI: 10.1093/aob/mcn016 |
| Web URL(s):
https://academic.oup.com/aob/article/101/6/845/184667/Development-of-a-Genomic-Microsatellite-Library-in
Last checked: 03/02/2017
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2209341a |
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