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| Web URL(s): | http://www.actahort.org/books/783/783_30.htm
Last checked: 06/10/2008
Access conditions: Item is within a limited access website |
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| Publication Type:
| Report |
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| Author(s): | Zeng, Bing;
Zhang, Xin-Quan;
Lan, Ying |
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| Title: | Assessment of genetic diversity among Dactylis glomerata L. as determined by RAPD and SRAP |
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| Meeting Info.: | Proceedings of the II International Conference on Turfgrass Science and Management for Sports Fields: Beijing, China, June 24-29, 2007. |
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| Source: | Acta Horticulturae. Vol. 783, February 2008, p. 291-302. |
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| Publishing Information: | Leuven, Belgium: International Society for Horticultural Science |
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| # of Pages: | 12 |
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| Keywords: | TIC Keywords: Genetic diversity; Dactylis glomerata; Random amplified polymorphic DNA markers; Sequence-related amplified polymorphism; Molecular markers; Cultivar variation; Genetics
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| Abstract/Contents: | "SRAP (Sequence-related amplified polymorphism) and RAPD (Random Amplified Polymorphic DNA) are two advanced molecular markers for genetic research in turfgrass. The present study represented the first report that evaluated the levels and patterns of genetic diversity among 45 accessions of Dactylis glomerata L obtained from eight geographically and environmentally distinct countries and regions. Twenty-one pairs of primer were used and 480 bands were produced in SRAP, of which 405 (84.38%) were polymorphic. On the other hand, twenty primers were used and 120 bands were produced in RAPD, of which 97 (80.83%) were polymorphic. The genetic similarity coefficients varied from 0.6248-0.9686 and 0.6154-0.9254, respectively. The genetic diversity of D. glomerata L. from China and America was richer than others. Based on cluster and principal component analysis on the genetic characteristics, all collections could be divided into four groups and five groups in two markers, respectively. Accessions from the identical continent were classified into the same group, indicating the geographical distribution of genetic diversity of D. glomerata L., which suggested that the genetic basis of cultivars is narrow, especially that in Chinese cultivars. The collection from Australia was different from other accessions in genetic diversity. Based on these results, SRAP and ISSR are considered useful tools for evaluating the genetic diversity for D. glomerata L., but SRAP detected more variance and gave clearer cluster groups. According to the analysis of genetic diversity and relationships, the appropriate strategies for collection and conservation of germplasm resources also were discussed." |
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| Language: | English |
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| References: | 0 |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zeng, B., X.-Q Zhang, and Y. Lan. 2008. Assessment of genetic diversity among Dactylis glomerata L. as determined by RAPD and SRAP. Acta Horticulturae. 783:p. 291-302. |
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| Web URL(s):
http://www.actahort.org/books/783/783_30.htm
Last checked: 06/10/2008
Access conditions: Item is within a limited access website |
| 
MSU catalog number: SB 13 .A25 no. 783 |
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