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Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2008.98.6.S9#page=21 Last checked: 10/20/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Caasi, O. C.;
Walker, N. R.;
Marek, S. M.;
Mitchell, T. K. |
Author Affiliation: | Caasi, Walker and Marek: Oklahoma State University, Stillwater, Oklahoma; Mitchell: Ohio State University, Columbus, Ohio |
Title: | Evaluation of transgenic Ophiosphaerella herpotricha expressing green and red fluorescent proteins in turftype bermudagrass |
Source: | Phytopathology. Vol. 98, No. 6, June Supplement 2008, p. S29. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: Ophiosphaerella herpotricha; Spring dead spot; Cynodon dactylon; Cynodon transvaalensis; Fungal diseases
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Cultivar Names: | Tifway 419; Midlawn |
Abstract/Contents: | "Ophiosphaerella herpotricha is one of the causal agents of spring dead spot in turftype bermudagrass (Cynodon spp. and interspecific hybrids) in the transition zone of the United States. Isolates of O. herpotricha were transformed using Agrobacterium tumefaciens to express either green (GFP) or red (tdTomato) fluorescent protein. Two turftype cultivars, Tifway 419 and Midlawn and a Cynodon transvaalensis accession were used to study the infection and colonization of roots by O. herpotricha under controlled conditions. Roots were inoculated with fungal mycelium and plants were incubated at 17°C. At 2 DPI (days postinoculation), transverse and longitudinal sections revealed fungal penetration through intact root epidermal layers for all plants. At 10 DPI, O. herpotricha colonized root cortical tissues of Tifway 419 and Midlawn intracellularly, formed aggregated hyphae, and caused necrotic lesions and collapse of cortical cells. At 14 DPI, roots of Midlawn and Tifway 419 exhibited a range of necroses while roots of C. transvaalensis were much less necrotic and appeared tolerant to infection by O. herpotricha." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Caasi, O. C., N. R. Walker, S. M. Marek, and T. K. Mitchell. 2008. Evaluation of transgenic Ophiosphaerella herpotricha expressing green and red fluorescent proteins in turftype bermudagrass. Phytopathology. 98(6):p. S29. |
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| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2008.98.6.S9#page=21 Last checked: 10/20/2015 Requires: PDF Reader Notes: Item is within a single large file |
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