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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2008.98.6.S9#page=98
Last checked: 10/20/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Miller, G. L.;
Tredway, L. P |
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| Author Affiliation: | North Carolina State University |
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| Title: | Phylogenetic analysis of the ITS1 and ITS2 rDNA regions of Lycoperdaceae associated with fairy rings on golf putting greens |
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| Source: | Phytopathology. Vol. 98, No. 6, June Supplement 2008, p. S106. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Fairy rings; Lycoperdon; DNA; Gene expression
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| Abstract/Contents: | "Identification of fungi associated with fairy rings is based solely on morphology of basidiocarps, which often do not develop or do not reach maturity due to turf management practices. Genomic DNA was extracted from puffballs and mycelial cords collected from 5 bermudagrass and 4 bentgrass greens in FL, HI, NC, and SC exhibiting fairy ring symptoms. The ITS1 and ITS2 regions of rDNA were amplified and sequenced using the basidiomycetespecific primer set ITS1f/ITS4b. Phylogenetic analyses, including construction of a parsimony tree and Bremer partition support calculations, were performed on the two ITS regions of 24 fieldcollected isolates and 6 downloaded sequences from Genbank. Parsimony analysis revealed only one tree, having high bootstrap and Bremer supports at each node. Twentythree of the unknown puffball isolates clustered with Genbank accessions of either Vascellum pratense or Lycoperdon pusillum, not with Lycoperdon perlatum, which is often cited as a prevalent cause of fairy rings in golf course putting greens in the Southeast. One of the unknown isolates grouped in a separate haplotype from all others. Morphology of the gleba of five mature puffballs was also probative of fungal genera. Puffballs with a true capillitium has ITS sequences most similar to Lycoperdon pusillum, while puffballs without capillitial threads had sequences most similar to Vascellum pratense." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Miller, G. L., and L. P. Tredway. 2008. Phylogenetic analysis of the ITS1 and ITS2 rDNA regions of Lycoperdaceae associated with fairy rings on golf putting greens. Phytopathology. 98(6):p. S106. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2008.98.6.S9#page=98
Last checked: 10/20/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: b2219736a |
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