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| DOI: | 10.1614/WS-08-020.1 |
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| Web URL(s): | http://www.bioone.org/perlserv/?request=get-document&doi=10.1614%2FWS-08-020.1
Last checked: 08/07/2008
Access conditions: Item is within a limited-access website
http://www.bioone.org/doi/pdf/10.1614/WS-08-020.1
Last checked: 03/15/2010
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| Publication Type:
| Refereed |
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| Author(s): | Jasieniuk, Marie;
Ahmad, Riaz;
Sherwood, Anna M.;
Firestone, Jeffrey L.;
Perez-Jones, Alejandro;
Lanini, W. Thomas;
Mallory-Smith, Carol;
Stednick, Zachary |
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| Author Affiliation: | Perez-Jones and Mallory-Smith: Department of Crop and Soil Science, Oregon State University, Corvallis, Oregon; Stednick: Department of Plant Sciences, University of California, Davis, California |
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| Title: | Glyphosate-resistant Italian ryegrass (Lolium multiflorum) in California: Distribution, response to glyphosate, and molecular evidence for an altered target enzyme |
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| Section: | Physiology, chemistry, and biochemistry
Other records with the "Physiology, chemistry, and biochemistry" Section
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| Source: | Weed Science. Vol. 56, No. 4, July/August 2008, p. 496-502. |
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| Publishing Information: | Champaign, IL: Weed Science Society of America |
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| # of Pages: | 7 |
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| Keywords: | TIC Keywords: Lolium multiflorum; Glyphosate; Herbicide resistance; Geographical distribution; Biomass; Shikimic acid; Application rates; Gene mapping; Enzymes
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| Abstract/Contents: | "Selection by herbicides has resulted in widespread evolution of herbicide resistance in agricultural weeds. In California, resistance to glyphosate was first confirmed in rigid ryegrass in 1998. Objectives of this study were to determine the current distribution and level of glyphosate resistance in Italian ryegrass, and to assess whether resistance could be due to an altered target site. Seeds were sampled from 118 populations and seedlings were treated with glyphosate at 866 g ae ha-1. Percentage of survivors ranged from 5 to 95% in 54 populations. All plants from 64 populations died. One susceptible (S) population, four putatively resistant (R) populations, and one S accession from Oregon were used for pot doseresponse experiments, shikimic acid analyses, and DNA sequencing. Seedlings were treated with glyphosate at eight rates, ranging from 108 to 13,856 g ae ha-1. Shoot biomass was evaluated 3 wk after treatment and fit to a log-logistic regression equation. On the basis of GR50 (herbicide rate required to reduce growth by 50%) values, seedlings from putatively R populations were roughly two to 15 times more resistant to glyphosate than S plants. Shikimic acid accumulation was similar in all plants before glyphosate treatment, but at 4 and 7 DAT, S plants from California and Oregon accumulated approximately two and three times more shikimic acid, respectively, than R plants. Sequencing of a cDNA fragment of the EPSPS coding region revealed two different codons, both of which encode proline at amino acid position 106 in S individuals. In contrast, all R plants sequenced exhibited missense mutations at site 106. Plants from one population revealed a mutation resulting in a proline to serine substitution. Plants from three R populations exhibited a mutation corresponding to replacement of proline with alanine. Our results indicate that glyphosate resistance is widespread in Italian ryegrass populations of California, and that resistance is likely due to an altered target enzyme." |
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| Language: | English |
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| References: | 36 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Jasieniuk, M., R. Ahmad, A. M. Sherwood, J. L. Firestone, A. Perez-Jones, W. T. Lanini, et al. 2008. Glyphosate-resistant Italian ryegrass (Lolium multiflorum) in California: Distribution, response to glyphosate, and molecular evidence for an altered target enzyme. Weed Sci. 56(4):p. 496-502. |
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| DOI: 10.1614/WS-08-020.1 |
| Web URL(s):
http://www.bioone.org/perlserv/?request=get-document&doi=10.1614%2FWS-08-020.1
Last checked: 08/07/2008
Access conditions: Item is within a limited-access website
http://www.bioone.org/doi/pdf/10.1614/WS-08-020.1
Last checked: 03/15/2010
Requires: PDF Reader
Access conditions: Item is within a limited access website |
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MSU catalog number: b2203399a |
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