| |
| Publication Type:
| Refereed |
|---|
| Author(s): | Liu, Z.-W.;
Jarret, R. L.;
Kresovich, S.;
Duncan, R. R. |
|---|
| Author Affiliation: | Liu, Jarret, and Kresovich: Plant Genetic Resources, Agricultral Research Service, United States Department of Agriculture, Griffin,Georgia; Duncan: Department of Crop and Soil Services, University of Georgia, Georgia Experiment Station, Griffin, Georgia |
|---|
| Title: | Characterization and analysis of simple sequence repeat (SSR) loci in seashore paspalum (Paspalum vaginatum Swartz) |
|---|
| Source: | TAG: Theoretical and Applied Genetics. Vol. 91, No. 1, 1995, p. 47-52. |
|---|
| Publishing Information: | Berlin: Springer |
|---|
| # of Pages: | 6 |
|---|
| Keywords: | TIC Keywords: Paspalum vaginatum; DNA; Alleles; Clones; Genetics; Hybrids; Molecular markers; Simple sequence repeats
|
|---|
| Abstract/Contents: | "A size-fractionated TaqI genomic library of seashore paspalum (Paspalum vaginatum Swartz) was screened for the presence of (GA)n and (CA)n simple sequence repeats (SSRs). A total of 54 clones with a positive signal were detected among 13,000 clones screened. Forty-seven clones having repeats of nā„3 were identified, of which 85% were perfect, 13% were imperfect and 2% were compound repeat sequences. Five of ten primer pairs synthesized to amplify selected loci resulted in a product in the expected size range and were subsequently used to examine SSR polymorphisms among 46 ecotypes of P. vaginatum. The number of alleles resolved on agarose or polyacrylamide gels were similar and ranged from 6 to 16 with an average of 14 per locus. Phenetic analysis of SSR polymorphisms revealed genetic relationships among the P. vaginatum ecotypes that were in general agreement with relationships detemined previously by RAPD analysis of the same plant materials. Further screening of the genomic library did not identify (AT)n, trimeric of tetrameric repeats. Hybridization of an (ATT)8 oligonucleotide probe to genomic DNA isolated from I. batatas, E. coli, Citrullis lanatus and P. vaginatum suggested that the P. vaginatum genome contained significantly fewer ATT repeats than either the I. batatas or C. lanatus genome." |
|---|
| Language: | English |
|---|
| References: | 34 |
|---|
| Note: | Figures
Tables
Graphs |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Liu, Z.-W., R. L. Jarret, S. Kresovich, and R. R. Duncan. 1995. Characterization and analysis of simple sequence repeat (SSR) loci in seashore paspalum (Paspalum vaginatum Swartz). Theor. Appl. Genet. 91(1):p. 47-52. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=138848 |
| If there are problems with this record, send us feedback about record 138848. |
| Choices for finding the above item: |
|---|
| 
MSU catalog number: QH 426 .T47 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
