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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Navarrete, Nadia;
Van Sambeek, J. W.;
Preece, John E.;
Gaffney, Gerald |
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| Author Affiliation: | Navarrete and Gaffney: Department of Forestry; Preece: Department of Plant and Soil Science; Van Sambeek: United States Department of Agriculture Forest Service, Southern Illinios University, Carbondale, Illinios |
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| Title: | An in vitro microplant bioassay for tall fescue allelopathy to clonal white ash |
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| Source: | HortScience. Vol. 24, 1989, p. 101. |
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| Publishing Information: | Alexandria, VA: American Society for Horticultural Science |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Clones; Rooting; Festuca arundinacea; Leaching; Soils
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| Abstract/Contents: | "Four clones of white ash (Fraxinus, americana L.) selected for rapid microshoot proliferation were used. Proliferating axillary shoots (20 mm long) were rooted by pulsing in MS medium with 20% sucrose, 0.7% Difco Bacto agar, 5μuM NAA, and 1μuM IBA for 8 days in darkness, then transferring to microplant medium containing MS with 1/4-strength macro-salts (1/4 MS), 10% sucrose, and 0.7% agar. When roots were visible (6-10 days after transfer to microplant medium), microplants were transferred to microplant medium containing tall fescue (Festuca elatior L.) extracts. Cut leaves from tall fescue in 21.6 liter pots in a greenhouse were prepared by grinding or soaking 10g (f.w.) and bringing volume in 100 ml liquid 1/4 MS. Soil leachates were also tested. Extracts were filtered, then serially diluted with microplant medium. After 4 weeks in vitro, primary and secondary root length were reduced by extracts from 5 and 10 g ground leaves per 100 ml medium. However, extracts from soaked, cut leaves stimulated root growth by 30% when applied at a rate of 5g per 100 ml." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Navarrete, Nadia, J. W. Van Sambeek, John E. Preece, and G. Gaffney. 1989. An in vitro microplant bioassay for tall fescue allelopathy to clonal white ash. HortScience. 24:p. 101. |
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