Full TGIF Record # 14146
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Web URL(s):http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/phyto78n12_1513.pdf#page=9
    Last checked: 10/15/2015
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Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Shane, W. W.; Nameth, S. T.
Author Affiliation:Ohio State University
Title:Monoclonal Antibodies for Diagnosis of Necrotic Ring Spot of Turfgrass.
Source:Phytopathology. Vol. 78, No. 12, December 1988, p. 1521.
Publishing Information:St. Paul, MN: American Phytopathological Society
Keywords:TIC Keywords: Ophiosphaerella korrae; Necrotic ring spot; Poa pratensis; Magnaporthe poae; Rhizoctonia; Fusarium; Gaeumannomyces
Abstract/Contents:"Rapid diagnosis of necrotic ring spot disease of Kentucky bluegrass is hindered by the lack of definitive symptoms and culture characteristics. Verification currently requires the production of the sexual stage--a process that takes >1 month in culture. Monoclonal antibodies were produced against mycelial homogenates of Leptosphaeria korrae (LK), strain ATCC 56289, for development of a diagnostic test. Spleen Cells, from immunized Balb/c mice, were fused with NS-1 myeloma cells and supernatants from the resultant hybridoma were screened by indirect ELISA. Eleven lines testing positive against LK56289 were subcloned and tested twice. A subclone was selected exhibiting positive reactions against 11 LK fungi such as Magnaporthe poae, Rhizoctonia spp., usarium spp., and Gaeumannomyces spp., or with healthy plant sap."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Shane, W. W., and S. T. Nameth. 1988. Monoclonal Antibodies for Diagnosis of Necrotic Ring Spot of Turfgrass.. Phytopathology. 78(12):p. 1521.
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Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/phyto78n12_1513.pdf#page=9
    Last checked: 10/15/2015
    Requires: PDF Reader
    Notes: Item is within a single large file
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