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Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/phyto78n12_1513.pdf#page=9 Last checked: 10/15/2015 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Shane, W. W.;
Nameth, S. T. |
Author Affiliation: | Ohio State University |
Title: | Monoclonal Antibodies for Diagnosis of Necrotic Ring Spot of Turfgrass. |
Source: | Phytopathology. Vol. 78, No. 12, December 1988, p. 1521. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
Keywords: | TIC Keywords: Ophiosphaerella korrae; Necrotic ring spot; Poa pratensis; Magnaporthe poae; Rhizoctonia; Fusarium; Gaeumannomyces
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Abstract/Contents: | "Rapid diagnosis of necrotic ring spot disease of Kentucky bluegrass is hindered by the lack of definitive symptoms and culture characteristics. Verification currently requires the production of the sexual stage--a process that takes >1 month in culture. Monoclonal antibodies were produced against mycelial homogenates of Leptosphaeria korrae (LK), strain ATCC 56289, for development of a diagnostic test. Spleen Cells, from immunized Balb/c mice, were fused with NS-1 myeloma cells and supernatants from the resultant hybridoma were screened by indirect ELISA. Eleven lines testing positive against LK56289 were subcloned and tested twice. A subclone was selected exhibiting positive reactions against 11 LK fungi such as Magnaporthe poae, Rhizoctonia spp., usarium spp., and Gaeumannomyces spp., or with healthy plant sap." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Shane, W. W., and S. T. Nameth. 1988. Monoclonal Antibodies for Diagnosis of Necrotic Ring Spot of Turfgrass.. Phytopathology. 78(12):p. 1521. |
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| Web URL(s): http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/phyto78n12_1513.pdf#page=9 Last checked: 10/15/2015 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: SB 599 .P48 |
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