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| Web URL(s): | http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/phyto78n12_1513.pdf#page=85
Last checked: 10/15/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Shelby, R. A.;
Kelley, V. C. |
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| Author Affiliation: | Auburn University |
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| Title: | A Comparison of Elisa Methods Using Combinations of Monoclonal and Polyclonal Antibodies to Detect the Endophyte of Tall Fescue. |
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| Source: | Phytopathology. Vol. 78, No. 12, December 1988, p. 1597. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| Keywords: | TIC Keywords: Festuca arundinacea; Antibodies; Endophytes; Acremonium coenophialum
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| Abstract/Contents: | "Rabbit polyclonal (RP) and mouse monoclonal (MM) antibodies were developed against mycelial homogenates of the tall fescue endophyte, Acremonium coenophialum. Initial screening of MM hybridoma cultures for specific antibody production utilized a modified indirect ELISA with infected and noninfected seed and plants as well as cultures of the target antigen and other nonrelated seed and plant contaminants. MM antibodies were not superior to RP antibodies in this test, however there was considerable variation in the performance of MM clones. Of the four immunoglobulin subclasses tested, IGG-1 was superior. Further enhancement of sensitivity was obtained when both RP and MM antibodies were used in a modified sandwich ELISA with a commercial anti-rabbit or anti-mouse conjugate." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Shelby, R. A., and V. C. Kelley. 1988. A Comparison of Elisa Methods Using Combinations of Monoclonal and Polyclonal Antibodies to Detect the Endophyte of Tall Fescue.. Phytopathology. 78(12):p. 1597. |
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| Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/phyto78n12_1513.pdf#page=85
Last checked: 10/15/2015
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: SB 599 .P48 |
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