Full TGIF Record # 14161
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Web URL(s):http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/phyto78n12_1513.pdf#page=101
    Last checked: 10/15/2015
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Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Tisserat, N.
Author Affiliation:Kansas State University
Title:Differentiation of Ophiophaerella Herpotricha and Leptosphaeria Korrae by Restriction Fragment Length Polymorphism Analysis.
Source:Phytopathology. Vol. 78, No. 12, December 1988, p. 1613.
Publishing Information:St. Paul, MN: American Phytopathological Society
Keywords:TIC Keywords: Ophiosphaerella korrae; Fungi; Spring dead spot; DNA; Cynodon
Abstract/Contents:"Restriction fragment length polymorphisms (RFLP) were used to distinguish Ophiosphaerella herpotricha (Oh) and Leptosphaeria korrae (Lk); these fungi are associated with spring dead spot of bermudagrass. Total DNA was extracted from isolates and digested with EcoRI or HindIII. DNA fragments were seperated by agarose gel electrophoresis and stained with ethidium bromide. Six of seven Lk isolates had an identical satellite DNA fragment pattern. None of the Oh isolates showed a similar banding pattern. A rDNA genomic clone from Armillaria mella is being used in Southern blot analysis of RFLP gels to differentiate Lk and Oh. This technique may be useful in identifying fungi associated with patch diseases of turfgrass which do not readily fruit in culture."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tisserat, N. 1988. Differentiation of Ophiophaerella Herpotricha and Leptosphaeria Korrae by Restriction Fragment Length Polymorphism Analysis.. Phytopathology. 78(12):p. 1613.
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Web URL(s):
http://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/phyto78n12_1513.pdf#page=101
    Last checked: 10/15/2015
    Requires: PDF Reader
    Notes: Item is within a single large file
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MSU catalog number: SB 599 .P48
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