Full TGIF Record # 142424
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Web URL(s):http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-92-5-0660
    Last checked: 11/21/08
Publication Type:
i
Refereed
Author(s):Vincelli, Paul; Tisserat, Ned
Author Affiliation:Vincelli: University of Kentucky, Lexington, Kentucky; Tisserat: Colorado State University, Fort Collins, Colorado
Title:Nucleic acid-based pathogen detection in applied plant biology
Source:Plant Disease. Vol. 92, No. 5, May 2008, p. 660-669.
Publishing Information:St. Paul, MN: American Phytopathological Society
# of Pages:10
Keywords:TIC Keywords: Disease identification; Nucleic acid; Polymerase chain reaction; Techniques; Pathology; Amplification fragment length polymorphisms; Restriction fragment length polymorphism; Genetic code; DNA amplification; Gene mapping; Technology; Genetics
Abstract/Contents:Discusses pathogen detection, stating that "nucleic acid (NA)-based techniques are widely recognized as some of the most powerful [and]...typically are rapid, specific, and highly sensitive." Profiles the NA amplification technique, PCR (polymerase chain reaction), explaining that "PCR is widely recognized as a highly sensitive technique; detection of the DNA from as little as a single spore is possible." Also profiles restriction fragment length polymophisms (RFLP), amplified fragment length polymorphism (AFLP), and oligonucleotide arrays as possible NA amplification techniques. Explains that "for fungal, bacterial, and nematodal pathogens, ribosomal RNA genes and intervening sequences are common targets for PCR amplification." Lists potential benefits of NA-based techniques, including "rapid detection and identification of pathogens"; "quantification of pathogen biomass in host tissue"; and "determining the geographic origin of...pathogen strains." Concludes that "rigorous application of the appropriate controls is requisite when using NA-based tools...obtaining multiple lines of evidence in crucial cases - is essential to the critical application of these tools."
Language:English
References:115
Note:Pictures, color
Figures
Tables
Graphs
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Vincelli, P., and N. Tisserat. 2008. Nucleic acid-based pathogen detection in applied plant biology. Plant Dis. 92(5):p. 660-669.
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Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-92-5-0660
    Last checked: 11/21/08
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