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| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2009.pdf#page=15
Last checked: 06/03/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Zhang, Yan;
Liang, Cuiyue;
Xu, Yan;
Gianfagna, Thomas;
Huang, Bingru |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Evaluation of transgenic creeping bentgrass with the IPT gene for cytokinin synthesis under nitrogen or phosphorus deficiency |
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| Section: | Plenary presentations
Other records with the "Plenary presentations" Section
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| Meeting Info.: | New Brunswick, NJ: January 12, 2009 |
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| Source: | Proceedings of the Eighteenth Annual Rutgers TurfgrassSymposium. 2009, p. 14. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Transgenic turfgrasses; Cytokinins; Nutrient deficiency; Senescence; Gene expression
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| Abstract/Contents: | "Nutrient deficieny has been associated with leaf senescence Cytokinins (CKs) play critical roles in delaying leaf senescence. The objective of the study was to determine whether over-expression of a gene from Agrobacterium tumefaciens controlling CK biosynthesis, isopentenyl transferase (ipt), in a cool-season grass species, creeping bentgrass (Agrostis stolonifera L.), would delay or suppress leaf senescence under nitrogen (N) and phosphorus (P) deficiency. The ipt gene was ligated to a senescence-associated promoter, SAG12, and transferred into creeping bentgrass using Agrobacterium-mediated techniques. Plants of a SAG12:ipt transgenic like (S41) and a null transgenic line (control) were grown in nutrient solutions with all essential nutrients or without N or P for 21 d. Significant declines in leaf photochemical efficiency (Fv/Fm) and chlorophyll content of mature leaves were detected in both the control line and SAG12:ipt plants exposed to N deficiency, but not in the P deficiency treatment. Compared to the control line, SAG12:ipt plants had higher levels of Fv/Fm, chlorophyll content, and cytokinins (ZR, DHZR, and iPA) content in leaves under either P or N deficiency. These differences between the control line and SAG12:ipt line became more pronounced with treatment duration. Higher levels of nitrate reductase and acid phosphatase activities in leaves and roots were maintained in SAG12:ipt line compared to the control line at 21 d of treatment, suggesting that the transgenic plant was able to use the limited nutrients more efficiently. Despite an almost 50% decline on total leaf protein 7 d after the onset of nutrient deficiency treatments, ipt expression continued in the SAG12:ipt plants. The changes in ipt gene expression in the transgenic line were correlated with leaf chlorophyll content, and indicated that the expression of SAG12:ipt in creeping bentgrass was associated with delayed leaf senescence under nutrient deficiency." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhang, Y., C. Liang, Y. Xu, T. Gianfagna, and B. Huang. 2009. Evaluation of transgenic creeping bentgrass with the IPT gene for cytokinin synthesis under nitrogen or phosphorus deficiency. Proc. Annu. Rutgers Turfgrass Symp. p. 14. |
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| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2009.pdf#page=15
Last checked: 06/03/2016
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b3696858 |
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Digitally in TIC by file name: rutsy2009jan |
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