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| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2009.pdf#page=16
Last checked: 06/03/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Huang, Bingru;
Xu, Chenping;
Tian, Jiang;
Xu, Jichen;
Belanger, Faith |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Identification of proteins and genes associated with heat tolerance in Agrostis species |
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| Section: | Plenary presentations
Other records with the "Plenary presentations" Section
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| Meeting Info.: | New Brunswick, NJ: January 12, 2009 |
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| Source: | Proceedings of the Eighteenth Annual Rutgers TurfgrassSymposium. 2009, p. 15. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Heat resistance; Agrostis; Cultivar improvement; Proteins; Gene expression; Agrostis scabra; Gene mapping
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| Abstract/Contents: | "Summer Bentgrass Decline caused by heat stress is a common problem in creeping bentgrass (Agrostis stolonifera) greens. The current commercially available cultivars do not have adequate heat tolerance. Therefore, there is an urgent need to develop high quality creeping bentgrass with improved heat tolerance. The genetic mechanisms of heat tolerance in turfgrasses are not clear. Two independent studies, differential gene expression analysis and proteomic profiling, were conducted to explore the molecular mechanisms of higher heat tolerance of A. scabra vs. creeping bentgrass at both RNA and protein levels. Our research on the differential heat response of two Agrostis grass species indicated that A. scabra, the thermal Agrostis species closely related to creeping bentgrass, was able to maintain much higher quality and physiological functions for extended periods of time compared to creeping bentgrass under high soil temperatures. A total of 143 unique genes with higher expression level in heat-stressed thermal A. scabra wwere identified using differential display (DD-PCR) ot suppression subtractive hybridization (SSH) approaches. Proteomic profiling of heat-stressed vs. un-stressed A. scabra and creeping bentgrass has revealed 70 different proteins. A large portion of identified genes have functions involved in protein and carbon metabolism, signaling/transcription, and stress/defense and, therefore, are potentially associated with the higher heat tolerance of thermal A. scabra. Several common genes with known functions in heat tolerance were identified from carbon metabolism (fructose 1,6-bisphosphate aldolase), stress and defense responses (phenylalanine ammonia-lyase, disulfide isomerase, and glutathione S-transferase). All of these genes have suggested functions involved in heat stress tolerance by either increased glucose utilization or higher level of signaling molecules biosynthesis. In addition, four heat-responsive genes encoding heat shock protein 70 (HSP70), heat shock protein 16 (HSP16), cysteine protease (AsCP1), and expansion (AsEXP1) were selected based on their heat inducible patterns in both gene expression and Western analyses, and availability of the full length cDNA sequences for a higher chance of primer design. These genes may play critical roles in heat tolerance, and could be used as potential candidate genes for marker development." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Huang, B., C. Xu, J. Tian, J. Xu, and F. Belanger. 2009. Identification of proteins and genes associated with heat tolerance in Agrostis species. Proc. Annu. Rutgers Turfgrass Symp. p. 15. |
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| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2009.pdf#page=16
Last checked: 06/03/2016
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b3696858 |
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