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| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2009.pdf#page=46
Last checked: 06/03/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Leanzo, Joseph;
Chackalamannil, Annie;
Zilinskas, Barbara A. |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Genetic improvement of switchgrass as a biofuel feedstock |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, NJ: January 12, 2009 |
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| Source: | Proceedings of the Eighteenth Annual Rutgers TurfgrassSymposium. 2009, p. 45. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Cultivar improvement; Calli; Variety trials; Biofuels; Panicum virgatum; Agrobacterium tumefaciens
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| Abstract/Contents: | "The ongoing project is to develop an Agrobacterium-mediated transformation system for superior high yielding ecotypes of switchgrass that grow on marginal lands in the northern geographical region of the United States and southern Canada for the production of stable transgenic plants. The germplasm that we are using is that identified by Dr. Stacy Bonos and colleagues at Rutgers University to be adapted to the temperate region of the US and which exhibits increased biomass, modified/lower lignin content and improved tolerance to poor soils, disease and lodging. We were generously provided with mature seed harvested from five different ecotypes, namely Carthage, Contract, Kanlow, NSU and Timber. We compared these ecotypes with regard to ease of initiation of callus and propagation of embryogenic callus cultures. We found that Timber BBL-95316-1, a selection from a lowland ecotype, and NSU EB-6 9184-4, a selection from an upland ecotype, were most amenable for establishment and proliferation of callus in culture. In the past six months, we defined optimum conditions for production of embryogenic callus from these two ecotypes. In addition, we have empirically determined the ideal conditions for regeneration of plantlets from these callus cultures. We are in the process of defining the culture medium for production of the type of callus that has previously proven most successful for Agrobacterium-mediated transformation of turfgrass species. We are also performing a kill curve with callus produced from these two ecotypes to determine the appropriate concentration of the herbicide to be used as a selection agent for transformed cells. When we have amassed a sufficient amount of callus, we will conduct transformation trials using protocols that have been developed in our laboratory and modified as necessary for the species in hand. The ultimate goal is to genetically modify switchgrass cultivars, improved by Dr. Bonos and her colleagues through conventional breeding, for use as a biofuel feedstock." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Leanzo, J., A. Chackalamannil, and B. A. Zilinskas. 2009. Genetic improvement of switchgrass as a biofuel feedstock. Proc. Annu. Rutgers Turfgrass Symp. p. 45. |
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| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2009.pdf#page=46
Last checked: 06/03/2016
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b3696858 |
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Digitally in TIC by file name: rutsy2009jan |
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