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| Web URL(s): | http://turf.rutgers.edu/research/abstracts/symposium2009.pdf#page=55
Last checked: 06/03/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Torres, Mónica S.;
Singh, Ajay P.;
Shah, Sneha;
Herrera-Carrillo, Zazil;
Gianfagna, Thomas;
White, James F. Jr.;
Vorsa, Nicholi |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University; Singh and Vorsa: Philip E. Marucci Center for Blueberry and Cranberry Research and Extension, Rutgers University; Herrera-Carrillo: Cytopathology Environmental Laboratory, National School of Biological Sciences, National Polytechnic Institute, Santo Tomas, Mexico |
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| Title: | LC-MS-MS identification and quantification of phenolics in symbiotic tall fescue |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, NJ: January 12, 2009 |
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| Source: | Proceedings of the Eighteenth Annual Rutgers TurfgrassSymposium. 2009, p. 54. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Cook College, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Phenols; Symbiosis; Festuca arundinacea; Gene mapping; Flavonoids
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| Abstract/Contents: | "An estimated 20 to 30% of grass species are associated with systemic fungal endophytes. Endophytic fungi have been demonstrated to confer benefits to the host plant through production of alkaloids and related fungal metabolites leading to protection of the host from herbivory by mammals and insects that results in improved plant performance. Most studies of herbivory by mammals and insects that results in improved plant performance. Most studies of the chemistry of the grass-endophyte symbiosis have been focused on fungal metabolites such us ergot alkaloids and anti-herbivore functions. However, there is little understanding of the effects of the symbiosis on plant metabolites. Plant phenolics are a diverse group of aromatic molecules playing key roles in signaling between plants and microbes, in defense as antimicrobial agents, feeding deterrents, UV protection, and oxidation protection. HPLC coupled electrospray mass spectrometry in tandem mode (LC-ESI-MS-MS) with negative ion detection was used to generate a phenolic profile that was identified and quantified using standards, mass fragmentation patterns including mass spectra in full scan mode (MS), and MS/MS (i.e. product ion, precursor and neutral loss). Flavonoid concentrations in E+ plants (133.62 ug/g fresh weight^D+-28.7) was higher than in E- plants (58.92ug/g fresh weigh^D+-3.69), flavonoid composition was also different. Identified compounds included (1) 3-caffeoylquinic acid, (2) 5-caffeoylquinic acid, (3) 4-caffeoyliquinic acid (4)p-Coumaric acid-O-glucoside, (5) Caffeicacid-O-glucoside, (6) Quercetin-3-dirutinoside, (7) Quercetin-3-glucoside, (8) Quercetin-3-rutinoside, (9) Quercetin-3-dirutinoside, (10) Luteoline-7-O-galactoside, (11) Luteoline-7-O-glucoside (12) Kaempferol-O-glucoside, (13) Triacin, (14) apigenin C arabinosyl-O-glucoside (15) dimetoxy-luteolin-glucosyl-diferulate and other unidentified flavonoids and phenolic acids. Deprotonated aglycones were formed by loss of galactoside, glucoside and caffeoyllate residues from their glycosides. Fragmentation patterns demonstrated several derivatives; aglycons and phenolics provided characteristic ions for each flavonoid family. Further studies will need to determine the final structural elucidations." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Torres, M. S., A. P. Singh, S. Shah, Z. Herrera-Carrillo, T. Gianfagna, J. F. Jr. White, et al. 2009. LC-MS-MS identification and quantification of phenolics in symbiotic tall fescue. Proc. Annu. Rutgers Turfgrass Symp. p. 54. |
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| Web URL(s):
http://turf.rutgers.edu/research/abstracts/symposium2009.pdf#page=55
Last checked: 06/03/2016
Requires: PDF Reader
Notes: Item is within a single large file |
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MSU catalog number: b3696858 |
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