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| DOI: | 10.11111/j.1469-8137.2008.02409.x |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2008.02409.x/full
Last checked: 01/31/2014
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http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2008.02409.x/pdf
Last checked: 01/31/2014
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| Publication Type:
| Refereed |
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| Author(s): | Hisano, Hiroshi;
Kanazawa, Akira;
Yoshida, Midori;
Humphreys, Mervyn O.;
Iizuka, Masaru;
Kitamura, Keisuke;
Yamada, Toshihiko |
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| Author Affiliation: | Hisano, Kanazawa, and Kitamura: Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan; Yoshida: National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Sapporo, Japan; Humphreys: Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Cerdigion, United Kingdom; Iizuka: Graduate School of Science, Osaka City University, Sugimoto, Osaka, Japan; Yamada: Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, Japan |
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| Title: | Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass |
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| Section: | Research
Other records with the "Research" Section
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| Source: | New Phytologist. Vol. 178, No. 4, June 2008, p. 766-780. |
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| Publishing Information: | Oxford, England: Blackwell Publishing, for the New Phytologist Trust |
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| # of Pages: | 15 |
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| Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2008.02409.x/abstract
Last checked: 01/31/2014
Notes: Abstract only |
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| Keywords: | TIC Keywords: Gene expression; Lolium perenne; Temperatures; Enzymes; Fructans; Gene mapping; Carbohydrates; Fructan synthesis; Genetic code; Temperature response; Cold resistance; DNA; Ribonucleic acid
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| Abstract/Contents: | "Fructan is the major nonstructural carbohydrate reserve in temperate grasses. To understand regulatory mechanisms in fructan synthesis and adaptation to cold environments, the isolation, functional characterization and genetic mapping of fructosyltransferase (FT) genes in perennial ryegrass (Lolium perenne) are described. Six cDNAs (prft1-prft6) encoding FTs were isolated from cold-treated ryegrass plants, and three were positioned on a perennial ryegrass linkage map. Recombinant proteins were produced in Pichia pastoris and enzymatic activity was characterized. Changes in carbohydrate levels and mRNA levels of FT genes during cold treatment were also analysed. One gene encodes sucrose-sucrose 1-fructosyltransferase (1-SST), and two gene encode fructan-fructan 6G-fructosyltransferase (6G-FFT). Protein sequences for the other genes (prfts 1, 2 and 6) were similar to sucrose-fructan 6-fructosyltransferase (6-SFT). The 1-SST and prft1 genes were colocalized with an invertase gene on the ryegrass linkage map. The mRNA levels of prft1 and prft2 increased gradually during cold treatment, while those of the 1-SST and 6G-FFT genes first increased, but then decreased before increasing again during a longer period of cold treatment. Thus at least two different patterns of gene expression have developed during the evolution of functionally diverse FT genes, which are associated in a coordinated way with fructan synthesis in a cold environment." |
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| Language: | English |
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| References: | 50 |
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| Note: | Figures
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hisano, H., A. Kanazawa, M. Yoshida, M. O. Humphreys, M. Iikuka, K. Kitamura, et al. 2008. Coordinated expression of functionally diverse fructosyltransferase genes is associated with fructan accumulation in response to low temperature in perennial ryegrass. New Phytol. 178(4):p. 766-780. |
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| DOI: 10.11111/j.1469-8137.2008.02409.x |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2008.02409.x/full
Last checked: 01/31/2014
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2008.02409.x/pdf
Last checked: 01/31/2014
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2219226 |
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