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| Web URL(s): | http://a-c-s.confex.com/crops/2008am/webprogram/Paper43460.html
Last checked: 02/20/2009 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Tan, Chengcheng;
Wu, Yanqi;
Taliaferro, Charles;
Anderson, Michael;
Tauer, Chuck;
Samuels, Timmy |
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| Author Affiliation: | Tan, Wu, Taliaferro, Anderson, and Samuels: Plant and Soil Sciences; Tauer: Natural Resource Ecology and Management, Oklahoma State University, Stillwater, Oklahoma |
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| Title: | Testing PCR amplification of bermudagrass DNA with mircosatellite primers from sorghum |
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| Section: | Graduate student poster competition: II. Diseases, genetics, physiology and technology (posters)
Other records with the "Graduate student poster competition: II. Diseases, genetics, physiology and technology (posters)" Section
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| Meeting Info.: | Houston, Texas: October 5-9, 2008 |
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| Source: | 2008 Joint Annual Meeting: [Abstracts][GSA/SSSA/ASA/CSSA/GCAGS/HGS]. 2008, p. 43460. |
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| Publishing Information: | [Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America; Boulder, CO: Geological Society of America; Austin, TX: Gulf Coast Association of Geological Societies with the Gulf Coast Section of SEPM; Houston, TX: Houston Geological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Polymerase chain reaction; Cynodon; Sorghum bicolor; Simple sequence repeats; DNA amplification
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| Abstract/Contents: | "Bermudagrass (Cynodon sp.) is a warm-season, perennial species widely used for turf and forage in the southern United States and other warm regions in the world. The objective of this study was to test by Polymerase Chain Reaction (PCR) amplification the transferability of sorghum, Sorghum bicolor (L.), simple sequence repeat (SSR) marker primers to bermudagrass genomic DNA. Genomic DNA samples from three bermudagrass accessions [C. transvaalensis Burtt-Davy 'T577' (2n=2x=18), C. dactylon (L.) Pers. 'Zebra' (2n=4x=36), and C. dactylon (L.) Pers. 'Tifton 10' (2n=6x=54)], and one sorghum accession 'Westland A Line' as a control, were extracted with Zymo Research, Plant/Seed DNA Extraction Kit. These DNA samples were PCR amplified and screened using 354 sorghum genomic SSR primer pairs with two replications. Amplified PCR products were visualized using a Li-Cor 4300 DNA Analyzer. Primer pairs were scored as a band being present or absent. Sixty-two percent (62%) of the sorghum primers amplified reproducible bands for the African bermudagrass accession T577, 34% for Tifton 10, and 32% for Zebra. The Westland A Line DNA was amplified with 88% of the primers. This investigation indicated that sorghum SSR primer pairs are an efficient source of molecular markers for bermudagrass." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tan, C., Y. Wu, C. Taliaferro, M. Anderson, C. Tauer, and T. Samuels. 2008. Testing PCR amplification of bermudagrass DNA with mircosatellite primers from sorghum. Joint Ann. Meet. p. 43460. |
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Last checked: 02/20/2009 |
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