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| Web URL(s): | http://a-c-s.confex.com/crops/2008am/webprogram/Paper44328.html
Last checked: 02/28/2009 |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Wang, Ying;
Jiang, Yiwei |
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| Author Affiliation: | Wang: Department of Agronomy, Purdue University, West Lafayette, Indiana; Jiang: Agronomy Department, Purdue University - Agronomy Department, West Lafayette, Indiana |
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| Title: | Ploidy level and DNA content determination of perennial ryegrass germplasm by using flow cytometry |
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| Section: | Graduate student poster competition: II. Diseases, genetics, physiology and technology (posters)
Other records with the "Graduate student poster competition: II. Diseases, genetics, physiology and technology (posters)" Section
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| Meeting Info.: | Houston, Texas: October 5-9, 2008 |
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| Source: | 2008 Joint Annual Meeting: [Abstracts][GSA/SSSA/ASA/CSSA/GCAGS/HGS]. 2008, p. 44328. |
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| Publishing Information: | [Madison, WI]: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America; Boulder, CO: Geological Society of America; Austin, TX: Gulf Coast Association of Geological Societies with the Gulf Coast Section of SEPM; Houston, TX: Houston Geological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: DNA; Gene mapping; Germplasm; Laser flow cytometry; Lolium perenne; Ploidy
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| Abstract/Contents: | "Perennial ryegrass (Lolium perenne L.) is a commonly used cool-season turfgrass species. Both diploid (2n=14) and tetraploid (2n= 28) perennial ryegrass materials exist in the germplasm collection. The objective of this study was to determine the ploidy level and DNA content of perennial ryegrass germplasm to establish a diploid natural mapping population for marker-trait association analysis. A total of 200 accessions of perennial ryegrass were obtained from the USDA National Plant Germplasm System (NPGS). Three independent samplings of young leaf tissues were conducted, and the DNA content was analyzed using the flow cytometry technique. Among the 200 accessions, 194 diploid and 6 tetraploid were identified. The average DNA content was 5.60 ± 0.21 pg/2C for the diploid and 11.45 ± 0.38 pg/2C for the tetraploid, respectively. DNA content in both the diploid and the tetraploid was correlated with chromosome numbers in the root tips counted under a microscope. The results provide important information for genetic research and cultivar improvement of perennial ryegrass." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, Y., and Y. Jiang. 2008. Ploidy level and DNA content determination of perennial ryegrass germplasm by using flow cytometry. Joint Ann. Meet. p. 44328. |
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Last checked: 02/28/2009 |
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