Full TGIF Record # 146348
Item 1 of 1
DOI:10.1007/s10529-006-9162-z
Web URL(s):https://link.springer.com/article/10.1007%2Fs10529-006-9162-z
    Last checked: 10/04/2017
Publication Type:
i
Refereed
Author(s):Hu, Qian; Nelson, Kimberly; Luo, Hong
Author Affiliation:Hu and Luo: Department of Genetics, Biochemistry and Life Science Studies, Clemson University, Clemson, South Carolina; Hu, Nelson, and Luo: HybriGene Incorporated, West Kingston, Rhode Island
Title:FLP-mediated site-specific recombination for genome modification in turfgrass
Source:Biotechnology Letters. Vol. 28, No. 22, November 2006, p. 1793-1804.
# of Pages:12
Publishing Information:[Kew, England: Science and Technology Letters]
Keywords:TIC Keywords: Agrostis stolonifera; Cell culture; Poa pratensis; Suspension cultures; Transgenes; Transgenic turfgrasses
Abstract/Contents:"To develop molecular strategies for gene containment in genetically modified (GM) turfgrass, we have studied the feasibility of using the FLP/FRT site-specific DNA recombination system from yeast for controlled genome modification in turfgrass. Suspension cell cultures of creeping bentgrass (Agrostis stolonifera L.) and Kentucky bluegrass (Poa pratensis) were co-transformed with a FLP recombinase expression vector and a recombination-reporter test plasmid containing β-glucuronidase (gusA) gene which was separated from the maize ubiquitin (ubi) promoter by an FRT-flanked blocking DNA sequence to prevent its transcription. GUS activity was observed in co-transformed cells, in which molecular analyses indicated that FLP-mediated excision of the blocking sequence had brought into proximity the upstream promoter and the downstream reporter gene, resulting in GUS expression. Functional evaluation of the FLP/FRT system using transgenic creeping bentgrass stably expressing FLP recombinase confirmed the observation in suspension cell culture. Our results indicate that FLP/FRT system is a useful tool for genetic manipulation of turfgrass, pointing to the great potential of exploiting the system to develop molecular strategies for transgene containment in perennials."
Language:English
References:47
Note:Pictures, color
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Hu, Q., K. Nelson, and H. Luo. 2006. FLP-mediated site-specific recombination for genome modification in turfgrass. Biotechnol. lett. 28(22):p. 1793-1804.
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DOI: 10.1007/s10529-006-9162-z
Web URL(s):
https://link.springer.com/article/10.1007%2Fs10529-006-9162-z
    Last checked: 10/04/2017
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MSU catalog number: b2322029~S1a
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