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Web URL(s):http://www.sciencedirect.com/science/article/pii/S0304423808003919
    Last checked: 02/07/2014
    Access conditions: Item is within a limited-access website
Publication Type:
Author(s):Yuan, Xuejun; Wang, Zhiyong; Liu, Jianxiu; She, Jianming
Author Affiliation:Yuan: College of Life Sciences, Ninjing Agricultural University; Yuan, Wang, and Liu: Grass Research Centre, Institute of Botany, Jiangsu Province & Chinese Academy of Sciences; She: Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Ninjinh, China
Title:Development of a plant regeneration system from seed-derived calluses of centipedegrass [Eremochloa ophiuroides (Munro.) Hack]
Source:Scientia Horticulturae. Vol. 120, No. 1, March 3 2009, p. 96-100.
# of Pages:5
Publishing Information:Amsterdam, Elsevier
Keywords:TIC Keywords: 2,4-D; Calli; Cultivar evaluation; Cytokinins; Eremochloa ophiuroides; Growth studies; NAA; Regeneration; Shoot growth
Cultivar Names:E-126
Trade Names:BAP
Abstract/Contents:"The objective of this study is to establish plant regeneration system with the seed of the new Chinese selection 'E-126' of centipedegrass [Eremochloa ophiuroides (Munro.) Hack] as explant. In present study, the following results were obtained: (1) The medium formulation most suitable for calluses induction was identified to be MS with 1.0 mg l-1 2,4-D + 30 g l-1 mannitol + 50 ml l-1 coconut milk and the ratio of calluses induction was 96.0%, including 5.2% of yellow granule calluses induction. The above medium formulation was adopted for subculture. (2) The rate of shoot regeneration from yellow granular calluses was 98.0% by MS optimum medium formulation with 2.0 mg l-1 KT + 50 ml l-1 coconut milk. The differentiated rate retained as high as 88.0% even after 5 times of subculture and 18.6% after 15 times of subculture. The optimum medium formulation for shoot growth was identified to be MS medium plus 2.0 mg l-1 BAP, 0.8 mg l-1 NAA and 50 ml l-1 coconut milk. (3) The optimum medium for shoot rooting was identified to be MS medium with 0.6 mg l-1 NAA + 50 ml l-1 coconut milk, and the rooting rate to be 98.0%. The survival rate of transplanted plantlets from tubes to basin with soil was 92.0%. In conclusion, the plant regeneration system was successfully developed in this study, which may provide basic reference for screening of somaclonal variants and genetic transformation of centipedegrass."
Note:Pictures, b/w
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Yuan, X., Z. Wang, J. Liu, and J. She. 2009. Development of a plant regeneration system from seed-derived calluses of centipedegrass [Eremochloa ophiuroides (Munro.) Hack]. Scientia Horticulturae. 120(1):p. 96-100.
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    Last checked: 02/07/2014
    Access conditions: Item is within a limited-access website
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