Publication Type:
i
Refereed
Author(s): Boucaud, J. ;
Bigot, J.
Author Affiliation: Laboratoire de Biochimie et Physiologie vegetale I.R.B.A., GS 79, Universite de Caen, F-14032 Caen Cedex, France.
Title: Changes in the Activities of Nitrogen Assimilation Enzymes of Lolium perenne L. During Regrowth After Cutting
Source: Plant and Soil . Vol. 114, No. 1, February 1989, p. 121-125.
Publishing Information: Dordrecht, Netherland: Martinus Nijhoff Publishers
Related Web URL: http://link.springer.com/article/10.1007/BF02203089 Last checked: 10/14/2015 Notes: Abstract only
Keywords: TIC Keywords: Lolium perenne ; Nitrogen ; Assimilation ; Enzymes ; Regrowth ; Cutting ; Nitrate reductase ; Glutamine
Abstract/Contents: "The activities of key enxymes involved in N assimilation were investigated after defoliation of 6-week-old ryegrass plants grown in water culture conditions. In a first experiment, nitrate reductase, glutamine synthetase and glutamate dehydrogenase activities were measured in roots, stubble and leaves on the day of cutting and at 7-day intervals over the following 5-week period of regrowth. Ammonia assimilation enzymes showed little change whereas the nitrate reductase activity sharply decreased 2 weeks after clipping. In a second experiment, the nitrate reductase activity was measured at 2- or 3-day intervals 1 week before and 3 weeks after clipping. In vivo and in vitro assays both showed an increasing activity in leaves up to 8 days after cutting while root activity decreased. The opposite changes then occurred and both organs recovered their initial nitrate reductase activity levels after 12-14 days of regrowth. These fluctuations in nitrate reductase activity were considered to be related to the capacity for C assimilation and the nitrate availability."
Language: English
References: 28
Note: Graphs
ASA/CSSA/SSSA Citation (Crop Science -Like - may be incomplete ): Boucaud, J., and J. Bigot. 1989. Changes in the Activities of Nitrogen Assimilation Enzymes of Lolium perenne L. During Regrowth After Cutting. Plant Soil. 114(1):p. 121-125.
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