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DOI: | 10.1094/PHYTO.2009.99.6.S1 |
Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2009.99.6.S1#page=2 Last checked: 06/09/2009 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Agindotan, B. O.;
Ahonsi, M. O.;
Domier, L. L.;
Gray, M. E.;
Bradley, C. A. |
Author Affiliation: | Agindotan, Ahonsi, Gray and Bradley: University of Illinois, Urbana, Illinois; Domier: United States Department of Agriculture - Agricultural Research Service, University of Illinois, Urbana, Illinois |
Title: | A method for the identification of RNA viruses of miscanthus and switchgrass |
Section: | Abstracts submitted for presentation at the 2009 APS annual meeting Other records with the "Abstracts submitted for presentation at the 2009 APS annual meeting" Section
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Meeting Info.: | Portland, Oregon: August 1-5, 2009 |
Source: | Phytopathology. Vol. 99, No. 6, June Supplement 2009, p. S2. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: Biomass; Disease identification; Miscanthus; Panicum virgatum; Rhizomes; Ribonucleic acid; Viruses
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Abstract/Contents: | "Miscanthus x giganteus and Panicum virgatum (switchgrass) are two potential biomass crops being evaluated for cellulosic ethanol production. Viral diseases are potentially significant threats to these crops. M. x giganteus is sterile, and is vegetatively propagated through the use of rhizomes, thus biomass production could be reduced by spread of viruses by planting infected rhizomes. Therefore, identification of viruses infecting these crops is important for quarantine purposes, virus resistance breeding, and production of virus-free planting materials. Here, we report a new generic method that was used to detect known and unknown RNA viruses. The method involves partial virus purification of viruses from infected leaf tissue, nuclease digestion of unenecapsidated nucleic acids, extraction of encapsidated viral RNA, random amplification, cloning, sequencing of amplicons, and BLAST searches for similar sequences in GenBank. Using this method, we identified a possibly new Marafivirus in P. virgatum that is 76% identical to the nucleotide sequence of Maize rayado fino virus (MRFV), its closest relative in GenBank. Sugarcane mosaic virus (SCMV) from M. x giganteus and Maize dwarf mosaic virus (MDMV) from Johnsongrass also were identified. The method is being optimized for the detection of additional RNA and DNA viruses from different genera and families." |
Language: | English |
References: | 0 |
Note: | "2009 APS Annual Meeting" This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Agindotan, B. O., M. O. Ahonsi, L. L. Domier, M. E. Gray, and C. A. Bradley. 2009. A method for the identification of RNA viruses of miscanthus and switchgrass. Phytopathology. 99(6):p. S2. |
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| DOI: 10.1094/PHYTO.2009.99.6.S1 |
| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2009.99.6.S1#page=2 Last checked: 06/09/2009 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b2219736a |
| Find from within TIC: Digitally in TIC by file name: phytp2009junpres |
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