| |
| DOI: | 10.1094/PHYTO.2009.99.6.S1 |
|---|
| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2009.99.6.S1#page=26
Last checked: 06/09/2009
Requires: PDF Reader
Notes: Item is within a single large file |
|---|
| Publication Type:
| Report |
|---|
| Content Type: | Abstract or Summary only |
|---|
| Author(s): | Cutulle, M. A.;
Horvath, B.;
McCall, D.;
Derr, J. |
|---|
| Author Affiliation: | Cutulle, Horvath and McCall: Virginia Tech, Ellicott City, Maryland; Derr: Virginia Tech, Virginia Beach, Virginia |
|---|
| Title: | Development of a chitinase assay for tall fescue challenged with Rhizoctonia solani |
|---|
| Section: | Abstracts submitted for presentation at the 2009 APS annual meeting
Other records with the "Abstracts submitted for presentation at the 2009 APS annual meeting" Section
|
|---|
| Meeting Info.: | Portland, Oregon: August 1-5, 2009 |
|---|
| Source: | Phytopathology. Vol. 99, No. 6, June Supplement 2009, p. S26-S27. |
|---|
| Publishing Information: | St. Paul, MN: American Phytopathological Society |
|---|
| # of Pages: | 2 |
|---|
| Keywords: | TIC Keywords: Chitinase; Disease resistance; Disease susceptibility; Festuca arundinacea; Inoculation; Rhizoctonia blight; Rhizoctonia solani; Visual evaluation
|
|---|
| Cultivar Names: | Matador; Kentucky 31; Jaguar |
|---|
| Abstract/Contents: | "Tall Fescue is a commonly utilized turfgrass in the transition zone of the U.S. However, it is susceptible to the fungal pathogen Rhizoctonia solani. Chitinase activity has been correlated with resistance to Rhizoctonia solani in several plants. We hope to develop a protocol for quantifying chitinase levels in tall fescue challenged with Rhizoctonia solani. Three tall fescue cultivars Matador, Kentucky 31, and Jaguar were inoculated with AG-2 141, Ag-2 146 strains of Rhizoctonia solani or non-inoculated control. Two hundred mg of shoot tissue was extracted 0 and 48 hours after inoculation from each sample. A bicinchoninic acid (BCA) colometricassay was utilized to quantify protein in the tissue extracts. Concurrently, tissue extracts were incubated with blue-dye labeled chitin and absorbance was measured. Finally, visual ratings for brown patch were taken one week after inoculation. Forty-eight hours post inoculation chitinase activity was greater in the Jaguar variety across both isolates when compared to the other two cultivars. Additionally, Jaguar showed less visible symptoms then the other cultivars. Based on these preliminary findings Jaguar expresses chitinase in an efficient manner i.e., when the plants senses the fungal pathogen (possibly senses chitin). The high expression of chitinase in Jaguar relative to other cultivars during the potential colonization period of Rhizoctonia solani may explain why Jaguar is more resistant then Matador or K31 to brown patch." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| Note: | This item is an abstract only!
"2009 APS Annual Meeting" |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Cutulle, M. A., B. Horvath, D. McCall, and J. Derr. 2009. Development of a chitinase assay for tall fescue challenged with Rhizoctonia solani. Phytopathology. 99(6):p. S26-S27. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=148961 |
| If there are problems with this record, send us feedback about record 148961. |
| Choices for finding the above item: |
|---|
| DOI: 10.1094/PHYTO.2009.99.6.S1 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2009.99.6.S1#page=26
Last checked: 06/09/2009
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b2219736a |
| Find from within TIC:
Digitally in TIC by file name: phytp2009junpres |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
