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DOI: | 10.1094/PHYTO.2009.99.6.S1 |
Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2009.99.6.s1#page=68 Last checked: 06/10/2009 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Lakshman, D. K.;
Natarajan, S. S.;
Garrett, W. M.;
Lakshman, S. |
Author Affiliation: | Lakshman, D. K.: United States Department of Agriculture - Agricultural Research Service, Beltsville, Maryland; Natarajan: United States Department of Agriculture - Agricultural Research Service, Soybean Genomics and Improvement Laboratory; Garrett: United States Department of Agriculture - Agricultural Research Service, Animal Biosciences and Biotechnology Laboratory; Lakshman, S.: United State Department of Agriculture - Agricultural Research Service, SIGL |
Title: | Global identification of cellular and excreted proteins of Rhizoctonia solani |
Section: | Abstracts submitted for presentation at the 2009 APS annual meeting Other records with the "Abstracts submitted for presentation at the 2009 APS annual meeting" Section
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Meeting Info.: | Portland, Oregon: August 1-5, 2009 |
Source: | Phytopathology. Vol. 99, No. 6, June Supplement 2009, p. S68. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: Identification; Protein extraction; Proteins; Rhizoctonia solani
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Abstract/Contents: | "Rhizoctonia solani, anastomosis group (AG) 4 (Tele: Thanatephorus cucumeris, T. praticola) is a soilborne and broad host range basidiomycetous fungus pathogenic to ornamentals, herbs, legumes, vegetables, turfgrasses and forest trees. Limited information on characterized genes and proteins of R. solani makes it difficult to carry out global genomic investigations to understand its biology and host-pathogen interactions. We earlier reported two cellular protein extraction methods optimized for R. solani. In this investigation, we have developed an extraction method and two-dimensional (2-D) gel resolution of excreted proteins of R. solani AG 4, isolate Rs23. We have detected over 50 secreted proteins from the (2-D) gel covering pH 4-7 and 6.5 - 205 kDa. In addition, 150 of the resolved, major and minor cellular protein spots were analyzed using MALDI-TOF-MS and LC-MS/MS, by comparing with cross-species fungal protein and Gene Ontology (GO) databases and with MASCOT (Matrix Science), BLAST (NCBI) and AmiGO (GO) search utilities. At least 140 protein spots were positively identified which could be associated with nucleus, mitochondria, cytosol, vacuole, plasma membrane, cell wall, and surface proteins. Identification of proteins even in absence of a homologous annotated genome database demonstrates the feasibility of carrying out R. solani protein profiling and pathogenic investigation." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! "2009 APS Annual Meeting" |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Lakshman, D. K., S. S. Natarajan, W. M. Garrett, and S. Lakshman. 2009. Global identification of cellular and excreted proteins of Rhizoctonia solani. Phytopathology. 99(6):p. S68. |
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| DOI: 10.1094/PHYTO.2009.99.6.S1 |
| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2009.99.6.s1#page=68 Last checked: 06/10/2009 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b2219736a |
| Find from within TIC: Digitally in TIC by file name: phytp2009junpres |
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