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DOI: | 10.1094/PHYTO.2009.99.6.S1 |
Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2009.99.6.S1#page=128 Last checked: 06/11/2009 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Tomaso-Peterson, M.;
Jo, Y. |
Author Affiliation: | Tomaso-Peterson: Mississippi State University, Mississippi State, Mississippi; Jo: Texas A&M University, College Station, Texas |
Title: | Determination of an etiological agent causing a novel foliar disease of zoysiagrass |
Section: | Abstracts submitted for presentation at the 2009 APS annual meeting Other records with the "Abstracts submitted for presentation at the 2009 APS annual meeting" Section
|
Meeting Info.: | Portland, Oregon: August 1-5, 2009 |
Source: | Phytopathology. Vol. 99, No. 6, June Supplement 2009, p. S128-S129. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 2 |
Keywords: | TIC Keywords: Exserohilum; Foliar diseases; Golf fairways; Hurricanes; Koch's postulates; Zoysia
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Cultivar Names: | Zeon |
Geographic Terms: | Tomball, Texas |
Abstract/Contents: | "A new foliar disease was observed on 'Zeon' zoysiagrass fairways at a golf course in Tomball, TX, following Hurricane Ike in October, 2008. Disease symptoms exhibited prominent elliptical black lesions along the margin of the leaves and leaf sheaths. Copious amounts of dark-gray mycelium were evident on the plant surface after incubation of the diseased sample at 22°C with high humidity for 3 days. The purpose of this study was to identify the etiological agent of this novel foliar disease of zoysiagrass and confirm pathogenicity using Koch's postulates. None of the eight-four isolates from symptomatic leaves produced a sporocarp in culture. Therefore, identification was accomplished through molecular means. Genomic DNA was extracted from a pure culture. The internal transcribed spacer region of ribosomal DNA was extracted from a pure culture. The internal transcribed spacer region of ribosomal DNA was amplified by PCR using ITS1 and ITS4 primers and was sequenced. The resultant DNA sequence was best matched with isolate NK03-1 of Exserohilum sp. (GenBank Accession no AB245152.1) with 99% identity. In Koch's postulates, foliar lesions were produced on zoysiagrass plants 21 days after zoysiagrass was seeded into soil infested with Exserohilum sp. The lesions were the same as those observed on the original diseased zoysiagrass sample from Texas. Exserohilum sp. was successfully reisolated. Confirmation of Exserohilum sp. as the etiological agent of a novel foliar disease of zoysiagrass was achieved." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! "2009 APS Annual Meeting" |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Tomaso-Peterson, M., and Y. Jo. 2009. Determination of an etiological agent causing a novel foliar disease of zoysiagrass. Phytopathology. 99(6):p. S128-S129. |
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| DOI: 10.1094/PHYTO.2009.99.6.S1 |
| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2009.99.6.S1#page=128 Last checked: 06/11/2009 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b2219736a |
| Find from within TIC: Digitally in TIC by file name: phytp2009junpres |
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