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DOI: | 10.1094/PHYTO.2004.94.6.S1 |
Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2004.94.6.S1#page=102 Last checked: 06/12/2009 Requires: PDF Reader Notes: Item is within a single large file |
Publication Type:
| Report |
Content Type: | Abstract or Summary only |
Author(s): | Thomas, S. L.;
Lipps, P. E.;
Boehm, M. J. |
Author Affiliation: | Thomas and Boehms: Ohio State University, Columbus; Lipps: Ohio State University, Wooster, Ohio |
Title: | Use of morphological, molecular, and pathogenic characteristics to identify Gaeumannomyces graminis varieties |
Section: | 2004 APS Annual Meeting abstracts of presentations Other records with the "2004 APS Annual Meeting abstracts of presentations" Section
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Meeting Info.: | Anaheim, California: July 31-August 4, 2004 |
Source: | Phytopathology. Vol. 94, No. 6S, June Supplement 2004, p. S102. |
Publishing Information: | St. Paul, MN: American Phytopathological Society |
# of Pages: | 1 |
Keywords: | TIC Keywords: Agrostis stolonifera; Gaeumannomyces graminis; Morphology; Pathogenicity; Take-all patch
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Abstract/Contents: | "Take-all, caused by Gaeumannomyces graminis, is a devastating root disease on cereal crops and turfgrasses worldwide. Three varieties of the fungus have been described and are distinguished from one another based on hyphopodium type and ascospore length. G. graminis var. graminis is readily differentiated from G. graminis var. avenae (Gga) and G. graminis var. tritici (Ggt) based on the production of lobed hyphopodia. Gga and Ggt produce simple hyphopodia and can only be distinguished morphologically from one another based on ascospore length. Although Gga has generally longer ascospores, most published ascospore length ranges contain a 5-10 µm overlap making it impossible to classify isolates with intermediate ascospore lengths. Pathogenicity on oats and variety-specific PCR primers can also be used to distinguish Gga from Ggt. The goal of this study was to compare the utility of using morphological, molecular and infectivity assays to classify isolates of G. graminis to the variety level. Seventeen of 38 isolates (45%) could be identified as either Gga or Ggt based solely on ascospore length. Molecular characterization using previously described variety-specific avenacinase or G. graminis-specific 18S rDNA primer sets allowed for Gga to be differentiated from Ggt 92% and 89% of the time, respectively. Pathogenicity on oats provided consistent and absolute (100%) differentiation of Gga and Ggt." |
Language: | English |
References: | 0 |
Note: | This item is an abstract only! |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Thomas, S. L., P. E. Lipps, and M. J. Boehm. 2004. Use of morphological, molecular, and pathogenic characteristics to identify Gaeumannomyces graminis varieties. Phytopathology. 94(6S):p. S102. |
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| DOI: 10.1094/PHYTO.2004.94.6.S1 |
| Web URL(s): http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2004.94.6.S1#page=102 Last checked: 06/12/2009 Requires: PDF Reader Notes: Item is within a single large file |
| MSU catalog number: b2219736a |
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