Full TGIF Record # 149253
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DOI:10.1094/PHYTO.2004.94.6.S1
Web URL(s):http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2004.94.6.S1#page=102
    Last checked: 06/12/2009
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Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Thomas, S. L.; Lipps, P. E.; Boehm, M. J.
Author Affiliation:Thomas and Boehms: Ohio State University, Columbus; Lipps: Ohio State University, Wooster, Ohio
Title:Use of morphological, molecular, and pathogenic characteristics to identify Gaeumannomyces graminis varieties
Section:2004 APS Annual Meeting abstracts of presentations
Other records with the "2004 APS Annual Meeting abstracts of presentations" Section
Meeting Info.:Anaheim, California: July 31-August 4, 2004
Source:Phytopathology. Vol. 94, No. 6S, June Supplement 2004, p. S102.
Publishing Information:St. Paul, MN: American Phytopathological Society
# of Pages:1
Keywords:TIC Keywords: Agrostis stolonifera; Gaeumannomyces graminis; Morphology; Pathogenicity; Take-all patch
Abstract/Contents:"Take-all, caused by Gaeumannomyces graminis, is a devastating root disease on cereal crops and turfgrasses worldwide. Three varieties of the fungus have been described and are distinguished from one another based on hyphopodium type and ascospore length. G. graminis var. graminis is readily differentiated from G. graminis var. avenae (Gga) and G. graminis var. tritici (Ggt) based on the production of lobed hyphopodia. Gga and Ggt produce simple hyphopodia and can only be distinguished morphologically from one another based on ascospore length. Although Gga has generally longer ascospores, most published ascospore length ranges contain a 5-10 µm overlap making it impossible to classify isolates with intermediate ascospore lengths. Pathogenicity on oats and variety-specific PCR primers can also be used to distinguish Gga from Ggt. The goal of this study was to compare the utility of using morphological, molecular and infectivity assays to classify isolates of G. graminis to the variety level. Seventeen of 38 isolates (45%) could be identified as either Gga or Ggt based solely on ascospore length. Molecular characterization using previously described variety-specific avenacinase or G. graminis-specific 18S rDNA primer sets allowed for Gga to be differentiated from Ggt 92% and 89% of the time, respectively. Pathogenicity on oats provided consistent and absolute (100%) differentiation of Gga and Ggt."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Thomas, S. L., P. E. Lipps, and M. J. Boehm. 2004. Use of morphological, molecular, and pathogenic characteristics to identify Gaeumannomyces graminis varieties. Phytopathology. 94(6S):p. S102.
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DOI: 10.1094/PHYTO.2004.94.6.S1
Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2004.94.6.S1#page=102
    Last checked: 06/12/2009
    Requires: PDF Reader
    Notes: Item is within a single large file
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MSU catalog number: b2219736a
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