Full TGIF Record # 149255
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DOI:10.1094/PHYTO.2004.94.6.S1
Web URL(s):http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2004.94.6.S1#page=103
    Last checked: 06/12/2009
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Publication Type:
i
Report
Content Type:Abstract or Summary only
Author(s):Toda, T.; Mghalu, J. M.; Hayakawa, T.; Hyakumachi, M.
Author Affiliation:Toda, Mghalu and Hyakumachi: Gifu University, Gifu; Hayakawa: Riken Green Company Limited, Iwata, Japan
Title:Development of specific primers for each variety of Rhizoctonia circinata
Section:2004 APS Annual Meeting abstracts of presentations
Other records with the "2004 APS Annual Meeting abstracts of presentations" Section
Meeting Info.:Anaheim, California: July 31-August 4, 2004
Source:Phytopathology. Vol. 94, No. 6S, June Supplement 2004, p. S103.
Publishing Information:St. Paul, MN: American Phytopathological Society
# of Pages:1
Keywords:TIC Keywords: Disease identification; Rhizoctonia circinata; Symptoms
Abstract/Contents:"Four varieties of Rhizoctonia circinata; var. agrostis, var. circinata, var. oryzae and var. zeae, are the causal agents of turfgrass diseases during warm seasons. As their symptoms are quite similar, it is difficult to distinguish among these four varieties. Conventional identification technique based on the cultural morphology of the isolated pathogen is laborious and time-consuming. For rapid detection of each variety of R. circinata, specific primers were designed from the sequence data of internal transcribed spacers of ribosomal DNA (rDNA-ITS region). The primers were Agro-1F for R. c. var. agrostis, Cir-2F for R. c. var. circinata, Ory-1F for R. c. var. oryzae and Zea- 2F for R. c. var. zeae. Each primer pair of Agro-1F/ITS 4, Cir-1F/ITS 4, Ory- 1F/ITS 4 and Zea-2F/ITS 4 could respectively produce a single PCR product from pure fungal DNA for each variety of R. circinata. When the primers were tested on other fungal pathogens of turfgrass, they showed no PCR products. The primer pairs of Agro-1F/ITS 4, Cir-2F/ITS 4, Ory-1F/ITS 4 and Zea-2F/ITS 4 are specific and useful for the rapid detection of respective R. circinata varieties."
Language:English
References:0
Note:This item is an abstract only!
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Toda, T., J. M. Mghalu, T. Hayakawa, and M. Hyakumachi. 2004. Development of specific primers for each variety of Rhizoctonia circinata. Phytopathology. 94(6S):p. S103.
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DOI: 10.1094/PHYTO.2004.94.6.S1
Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2004.94.6.S1#page=103
    Last checked: 06/12/2009
    Requires: PDF Reader
    Notes: Item is within a large file
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MSU catalog number: b2219736a
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