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| DOI: | 10.1094/PHYTO.2004.94.6.S167 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2004.94.6.S167#page=1
Last checked: 06/15/2009
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Abril, M.;
Curry, K. J.;
Wedge, D. E.;
Smith, B. J. |
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| Author Affiliation: | Abril and Curry: University of Southern Mississippi, Hattiesburg, Mississippi; Wedge: United States Department of Agriculture-Agricultural Research Service, University, Mississippi; Smith: United States Department of Agriculture-Agricultural Research Service, Poplarville, Mississippi |
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| Title: | Development of standardized techniques for screening natural product fungicides |
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| Section: | 2004 Southern Division meeting abstracts
Other records with the "2004 Southern Division meeting abstracts" Section
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| Meeting Info.: | Tulsa, Oklahoma: February 15-17, 2004 |
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| Source: | Phytopathology. Vol. 94, No. 6S, June Supplement 2004, p. S167. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Fungicidal properties; Fungicide evaluation; Product evaluation
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| Abstract/Contents: | "We developed two standardized procedures that improve the reliability of in vitro fungicide testing using strains of Colletotrichum and Botrytis. They required different incubation temperatures, but all were grown under coolwhite fluorescent lights with a 12 h photoperiod. Fungi were grown on Roswell Park Memorial Institute medium 1640. We developed a protocol for standardizing spore germination times. A concentration of 105 conidia/ml, harvested after 10 to 14 days, was optimal. Higher concentrations inhibited germination. We washed conidia three times in sterile water, thus significantly reducing germination time. We developed a leaf-clearing technique to assess reproducible germination conditions using detached strawberry leaves. Small leaf pieces were placed overnight in a solution of 1:2 absolute ethanol:glacial acetic acid, stained with 0.05% acid fuchsin for two minutes, and water rinsed. The leaves were clear, viable conidia were bright red, and nonviable conidia were clear." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Abril, M., K. J. Curry, D. E. Wedge, and B. J. Smith. 2004. Development of standardized techniques for screening natural product fungicides. Phytopathology. 94(6S):p. S167. |
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| DOI: 10.1094/PHYTO.2004.94.6.S167 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2004.94.6.S167#page=1
Last checked: 06/15/2009
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b2219736a |
| Find from within TIC:
Digitally in TIC by file name: phytp2004junsoutherndiv |
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