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| Web URL(s): | http://www.msmbb.org.my/apjmbb/html111/pg51.pdf
Last checked: 06/16/2009
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Ho, Chai Ling;
Kulaveerasingam, Harikrishna;
Kandasamy, Kodi Isparan;
Chin, Chiew Foan;
Rahman, Siti Suhaila Abdul |
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| Author Affiliation: | Ho and Kulaveerasingam: Department of Biotechnology, Faculty of Food Science and Biotechnology, Universiti Putra Malaysia, Selangor; Kandasamy: Forest Plantation Division, Forest Research Institute Malaysia, Kuala Lumpur; Chin and Rahman: TropBio Sdn. Bhd., Puchong, Selangor, Malaysia |
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| Title: | Variation in turfgrasses demonstrated by Amplified Fragment Length Polymorphism (AFLP) |
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| Source: | Asia Pacific Journal of Molecular Biology andBiotechnology. Vol. 11, No. 1, 2003, p. 51-55. |
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| Publishing Information: | Kuala Lumpur, Malaysia : Malaysian Society for Molecular Biology & Biotechnology [and] the National Working Group on Biotechnology |
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| # of Pages: | 5 |
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| Keywords: | TIC Keywords: Amplification fragment length polymorphisms; Genetic variability; Cultivar identification; DNA; Comparisons; Classification
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| Abstract/Contents: | "The classification of turfgrass cultivars is difficult as it has mostly relied on morphological characteristics that are multigenic, quantitative, and susceptible to modification by environmental factors. In this study, we employed an Amplified Fragment Length Polymorphic (AFLP) technique to distinguish between several turfgrass samples (including two 'offtypes') that were collected from Malaysian golf courses in comparison to four Australian Cynodon samples. The results clearly demonstrated the applicability of AFLP as a method to identify and fingerprint turfgrass samples and their 'offtypes'. The agreement of the three dendrograms generated by AFLP, Arbitrary Primed Polymorphic DNA (AP-PCR) and Internal Transcribed Spacer (ITS) gives good confidence in the use of the AFLP data for identification and analysis of relationships between the Australian Cynodon samples. The occurrence of 'offtypes' at one of the Malaysian golf courses is possibly a result from somatic mutation (TD6) or sod contamination at planting (TW4)." |
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| Language: | English |
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| References: | 10 |
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| Note: | Pictures, b/w
Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ho, C. L., H. Kulaveerasingam, K. I. Kandasamy, C. F. Chin, and S. S. A. Rahman. 2003. Variation in turfgrasses demonstrated by Amplified Fragment Length Polymorphism (AFLP). Asia Pacific Journal of Molecular Biology andBiotechnology. 11(1):p. 51-55. |
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Last checked: 06/16/2009
Requires: PDF Reader |
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