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Web URL(s): | https://link.springer.com/article/10.1023%2FA%3A1024050720199 Last checked: 10/04/2017 |
Publication Type:
| Refereed |
Author(s): | Basu, Chhandak;
Luo, Hong;
Kausch, Albert P.;
Chandlee, Joel M. |
Author Affiliation: | Basu: Department of Plant Sciences, University of Rhode Island; Luo and Kausch: HybriGene Incorporated, West Kingston; Chandlee: Department of Cell and Moleculer Biology, Morrill Science Center, University of Rhode Island, Kingston, Rhode Island |
Title: | Transient reporter gene (GUS) expression in creeping bentgrass (Agrostis palustris) is affected by in vivo nucleolytic activity |
Source: | Biotechnology Letters. Vol. 25, 2003, p. 939-944. |
Publishing Information: | [Kew, England: Science and Technology Letters] |
# of Pages: | 6 |
Keywords: | TIC Keywords: Agrostis stolonifera; Nucleases; Transgene expression
|
Cultivar Names: | Penn A-4 |
Abstract/Contents: | "Leaf and callus tissues of a creeping bentgrass cultivar (Penn A4) had high nuclease activities that degraded exogenously added plasmid DNA. When callus tissue was incubated for 24 h with heparin, spermidine, aurintricarboxylic acid or polyethylene glycol, only heparin and spermidine were effective as in vitro nuclease inhibitors, protecting exogenously added plasmid DNA from degradation. When Β-glucuronidase (GUS) reporter gene activity was evaluated in heparin-treated (0.6%), 14-month old callus following microprojectile bombardment, GUS activity increased 1000-fold compared to equivalent aged untreated Penn A4 callus. Similar enhancement from heparin pretreatment (0.6% or 1.2%) was not observed in 6-month old callus. This is likely due to much higher activities of nuclease in the younger callus." |
Language: | English |
References: | 24 |
Note: | Figures Graphs |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Basu, C., H. Luo, A. P. Kausch, and J. M. Chandlee. 2003. Transient reporter gene (GUS) expression in creeping bentgrass (Agrostis palustris) is affected by in vivo nucleolytic activity. Biotechnol. lett. 25:p. 939-944. |
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| Web URL(s): https://link.springer.com/article/10.1023%2FA%3A1024050720199 Last checked: 10/04/2017 |
| MSU catalog number: b2322029 |
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