Full TGIF Record # 149630
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Web URL(s):https://link.springer.com/article/10.1023%2FA%3A1024050720199
    Last checked: 10/04/2017
Publication Type:
Author(s):Basu, Chhandak; Luo, Hong; Kausch, Albert P.; Chandlee, Joel M.
Author Affiliation:Basu: Department of Plant Sciences, University of Rhode Island; Luo and Kausch: HybriGene Incorporated, West Kingston; Chandlee: Department of Cell and Moleculer Biology, Morrill Science Center, University of Rhode Island, Kingston, Rhode Island
Title:Transient reporter gene (GUS) expression in creeping bentgrass (Agrostis palustris) is affected by in vivo nucleolytic activity
Source:Biotechnology Letters. Vol. 25, 2003, p. 939-944.
# of Pages:6
Publishing Information:[Kew, England: Science and Technology Letters]
Keywords:TIC Keywords: Agrostis stolonifera; Nucleases; Transgene expression
Cultivar Names:Penn A-4
Abstract/Contents:"Leaf and callus tissues of a creeping bentgrass cultivar (Penn A4) had high nuclease activities that degraded exogenously added plasmid DNA. When callus tissue was incubated for 24 h with heparin, spermidine, aurintricarboxylic acid or polyethylene glycol, only heparin and spermidine were effective as in vitro nuclease inhibitors, protecting exogenously added plasmid DNA from degradation. When Β-glucuronidase (GUS) reporter gene activity was evaluated in heparin-treated (0.6%), 14-month old callus following microprojectile bombardment, GUS activity increased 1000-fold compared to equivalent aged untreated Penn A4 callus. Similar enhancement from heparin pretreatment (0.6% or 1.2%) was not observed in 6-month old callus. This is likely due to much higher activities of nuclease in the younger callus."
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Basu, C., H. Luo, A. P. Kausch, and J. M. Chandlee. 2003. Transient reporter gene (GUS) expression in creeping bentgrass (Agrostis palustris) is affected by in vivo nucleolytic activity. Biotechnol. lett. 25:p. 939-944.
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    Last checked: 10/04/2017
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