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| DOI: | 10.1046/j.1439-0523.2001.00594.x |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1046/j.1439-0523.2001.00594.x/pdf
Last checked: 01/31/2014
Requires: PDF Reader
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1046/j.1439-0523.2001.00594.x/abstract
Last checked: 01/31/2014
Notes: Abstract only |
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| Publication Type:
| Report |
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| Author(s): | Bai, Y.;
Qu, R. |
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| Author Affiliation: | Department of Crop Science, North Carolina State University, Raleigh, North Carolina |
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| Title: | Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue |
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| Source: | Plant Breeding. Vol. 120, No. 3, June 2001, p. 239-242. |
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| Publishing Information: | Berlin: P. Parey |
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| # of Pages: | 4 |
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| Keywords: | TIC Keywords: Tissue culture; Festuca arundinacea; Thidiazuron; Seeds; Genetic transformation; Calli; Cytokinins; Regeneration
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| Cultivar Names: | Coronado |
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| Abstract/Contents: | "In an eort to optimize tissue culture responses of turf-type tall fescue for genetic transformation, the eects of five culture medium supplements on tissue culture responses were investigated with immature embryos and mature seeds of an elite cultivar, `Coronado', as explant 1 tissues. For both explants, calli induced on 6-benzylaminopurine (BAP)-containing medium had signicantly improved regeneration ability. The optimal concentration of BAP for the induction of regenerable callus from mature seeds was 0.1 mg/l. Thidiazuron also improved callus regeneration frequency of mature seeds. Casein hydrolysate, L-proline and myo-inositol improved callus induction of immature embryos but not mature seeds. They did not improve callus regeneration frequencies with either explant tissue. By simply slicing the mature seeds into two halves longitudinally, the callus induction frequencies, as well as the corresponding overall plant regeneration frequencies, were increased approximately three- to six-fold in all three combinations of 2,4-dichlo-rophenoxyacetic acid and BAP in callus induction media." |
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| Language: | English |
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| References: | 23 |
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| Note: | Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Bai, Y., and R. Qu. 2001. Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue. Plant Breed. 120(3):p. 239-242. |
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| DOI: 10.1046/j.1439-0523.2001.00594.x |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1046/j.1439-0523.2001.00594.x/pdf
Last checked: 01/31/2014
Requires: PDF Reader
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1046/j.1439-0523.2001.00594.x/abstract
Last checked: 01/31/2014
Notes: Abstract only |
| 
MSU catalog number: SB 119 .A1 Z4 |
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