Full TGIF Record # 150986
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Web URL(s):https://archive.lib.msu.edu/tic/its/articles/2009jou253.pdf
    Last checked: 10/18/2011
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Publication Type:
i
Refereed
Author(s):Miller, Gerald L. Jr.; Tredway, Lane P.
Author Affiliation:Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina
Title:Selective amplification of fairy ring fungi from infested putting green soils
Section:Diseases (plant pathology)
Other records with the "Diseases (plant pathology)" Section
Meeting Info.:Santiago, Chile: July 26-30 2009
Source:International Turfgrass Society Research Journal. Vol. 11, No. Part 1, 2009, p. 253-262.
Publishing Information:Madison, WI: International Turfgrass Society
# of Pages:10
Keywords:TIC Keywords: Golf greens; Fairy rings; DNA amplification; Disease identification
Abstract/Contents:"Identification of fairy ring fungi on golf putting greens is difficult and rests solely on the morphology of basidiocarps, which may not occur or are often damaged by agronomic practices. The objective of this study was to investigate the use of molecular techniques to identify fairy ring pathogens in infested soils to species level. Genomic DNA was extracted from five soil samples from putting greens located in North Carolina and Texas infested with Type I and II fairy rings. Ribosomal DNA was amplified using the basidiomycete-specific primers ITS1f and ITS4b and directly sequenced, yielding a consensus ITS sequence for four of the five samples. Four to eight cloned PCR fragments were sequenced per sample, and showed great similarity (99%) to directly sequenced ITS fragments. ITS sequences were compared with known isolates and with Genbank accessions using the online BLAST search engine. Phylogenetic analysis revealed ITS sequences from two sampled soil sites to be genetically similar to Vascellum curtisii (Berk,) Kriesel, a known fairy ring pathogen in Japan. A PCR based assay using species specific primers for the fairy ring fungi Vascellum curtisii and Bovista dermoxantha was also evaluated. Target sequences were amplified successfully using these primers from both extracted soil DNA and DNA from known isolates of Vascellum curtisii and Bovista dermoxantha, however several false positive amplifications also occurred. Due to this variability in PCR detection assays, sequencing of extracted soil DNA may be a more viable alternative for detecting and identifying fairy ring pathogens."
Language:English
References:32
See Also:Other items relating to: Fairy Rings
Note:Figures
Tables
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Miller, G. L., and L. P. Tredway. 2009. Selective amplification of fairy ring fungi from infested putting green soils. Int. Turfgrass Soc. Res. J. 11(Part 1):p. 253-262.
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https://archive.lib.msu.edu/tic/its/articles/2009jou253.pdf
    Last checked: 10/18/2011
    Requires: PDF Reader
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