| Abstract/Contents: | "Data are few on concentrations of nitrogen (N) in the cell wall and lignocellulose (neutral- and acid-detergent fibre (NDF and ADF), respectively) of herbage. Herbage N can be partitioned into neutral- and acid-detergent soluble and insoluble N to crudely estimate rapidly degradable (N soluble in neutral detergent), slowly degradable (neutral-detergent fibre N (NDFN) minus acid-detergent fibre N (ADFN), herein termed available fibre N (AFN), and indigestible N (ADFN) in the rumen. Our objective was to examine the effects of herbage species, maturity stage, and plant part on N in the NDF and ADF of lucerne (Medicago sativa L.), red clover (Trifolium pratense L.), timothy (Phleum pratense L.), and smooth bromegrass (Bromus inermis Leyss.). Herbage was harvested at four 2-week intervals during spring of 1984 and 1985. Leaves, stems and total herbage were analysed for total N, and N in NDF and ADF. Concentrations of N in ADF in leaves, stems, and total herbage of lucerne and clover were twice that of grass ADF. This may be due to greater lignin concentrations generally reported in legumes. Concentrations of NDFN in leaves of lucerne, clover, timothy, and bromegrass were 9.8, 26.4, 8.6, and 6.4g kg-1 of NDF, respectively, averaged over harvests and years, whereas stem concentrations were 4.2, 4.6, 3.2, and 2.4 g kg-1 of NDF, respectively. Leaf concentrations of ADFN in lucerne, clover, timothy, and bromegrass were 4.6, 5.3, 2.0, and 1.6 g kg-1 of ADF, respectively, averaged over harvests and years, whereas stem concentrations were 3.4, 3.3, 1.4, and 1.4 g kg-1 of ADF, respectively. As a proportion of the total N, NDFN and ADFN increased with plant maturity in leaves, stems, and herbage. In stems, 49% of NDFN was ADFN, whereas in leaves only 21% of the NDFN and ADFN. The larger portion of ADFN in stems probably reflects the larger proportion of lignified xylem and other structural tissues, which have a greater proportion of lignified secondary cell walls, whereas the greater proportion of NDFN in leaves may mirror the greater amount of mesophyll cells with primary cell walls. More than 80% of the total N in leaves, stems, and herbage was in the cell solubles, which may be rapidly ruminally degradable." |
|---|
