Full TGIF Record # 155357
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DOI:10.1007/978-0-387-79144-9_24
Web URL(s):https://link.springer.com/chapter/10.1007%2F978-0-387-79144-9_24
    Last checked: 10/04/2017
Publication Type:
i
Report
Author(s):Smith, Kevin F.; Dobrowolski, Mark P.; Cogan, Noel O. I.; Spangenberg, Germán C.; Forster, John W.
Author Affiliation:Smith: Primary Industries Research Victoria, Hamilton Centre, Hamilton, VIC3300, Australia, Molecular Plant Breeding Cooperative Research Centre, Australia; Dobrowolski: Primary Industries Research Victoria, Hamilton Centre, Hamilton, VIC3300, Australia, Molecular Plant Breeding Cooperative Research Centre, Australia; Cogan, Spangenberg and Forster: Primary Industries Research Victoria, Victorian AgriBiosciences Centre, La Trobe Research and Development Park, Bundoora, VIC 3038, Australia, Molecular Plant Breeding Cooperative Research Centre, Australia
Title:Utilizing linkage disequilibrium and association mapping to implement candidate gene based markers in perennial ryegrass breeding
Meeting Info.:5th International Symposium on the Molecular Breeding of Forage and Turf, Sapporo, Japan, 2007
Source:Molecular Breeding of Forage and Turf: The Proceedings of the 5th International Symposium on the Molecular Breeding of Forage and Turf. 2009, p. 259-274.
# of Pages:16
Publishing Information:New York, New York: Springer
Keywords:TIC Keywords: Alleles; Association mapping; Gene specific markers; Genetic analysis; Genetic linkage; Lolium perenne; Molecular markers; Selective breeding
Abstract/Contents:"Development of accurate high-throughput molecular marker systems such as SNPs permits evaluation and selection of favourable gene variants to accelerate elite varietal production. SNP discovery in perennial ryegrass has been based on PCR amplification and sequencing of multiple amplicons designed to scan all componets of the transcriptional unit. Full-length genes (with complete intron-exon structure and promoter information) corresponding to well-defined biochemical functions such as lignin biosynthesis and oligosaccharide metabolism are ideal for complete SNP haplotype determination. Multiple SNPs at regular intervals across the transcriptional unit were detected within and between the heterozygous parents and validated in the progeny of the F1(NA6 × AU6) genetic mapping family. Haplotype structures in the parental genotypes were defined and haplotypic abundance, structure and variation were assessed in diverse germplasm sources. Decay of LD to r2 values of c. 0.2 typically occurs over 500-3,000 bp, comparable with gene length and with little apparent variation between diverse, ecotypic and varietal population subgroups. Similar patterns were revealed as limited blocks of intragenic LD. The results are compatible with the reproductive biology of perennial ryegrass and the effects of large ancestral population size. This analysis provides crucial information to validate strategies for correlation of haplotypic diversity and phenotypic variation through association mapping."
ISBN:0387791434
ISBN-13:9780387791432
9780387791449
Language:English
References:44
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Smith, K. F., M. P. Dobrowolski, G. C. Spangenberg, J. W. Forster, and N. O. I. Cogan. 2009. Utilizing linkage disequilibrium and association mapping to implement candidate gene based markers in perennial ryegrass breeding. Molecular Breeding of Forage and Turf: The Proceedings of the 5th International Symposium on the Molecular Breeding of Forage and Turf. p. 259-274.
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DOI: 10.1007/978-0-387-79144-9_24
Web URL(s):
https://link.springer.com/chapter/10.1007%2F978-0-387-79144-9_24
    Last checked: 10/04/2017

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Accession number: 226280666
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