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| Web URL(s): | https://turf.rutgers.edu/research/abstracts/symposium2010.pdf#page=36
Last checked: 02/06/2017
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Ambrose, Karen;
Kerstetter, Randall A.;
White, James F.;
Belanger, Faith C. |
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| Author Affiliation: | Department of Plant Biology and Pathology, Rutgers University |
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| Title: | Deep transcriptome comparison of endophyte-free and endophyte-infected fine fescue |
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| Section: | Poster presentations
Other records with the "Poster presentations" Section
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| Meeting Info.: | New Brunswick, NJ: January 11, 2010 |
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| Source: | Proceedings of the Nineteenth Annual Rutgers TurfgrassSymposium. 2010, p. 36. |
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| Publishing Information: | New Brunswick, NJ: Center for Turfgrass Science, Rutgers, The State University of New Jersey |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Comparisons; Endophyte-infected plants; Evaluations; Festuca rubra; Host pathogen interaction system; Poa pratensis; Relationships
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| Abstract/Contents: | "It is well established that the symbiotic fungal endophytes of grasses confer numerous benefits to their hosts. However, the details of the interaction are largely unknown. One of the outstanding questions regarding the plant-endophyte relationship is what are the factors contributing to maintenance of a compatible interaction. Understanding this may ultimately lead to the ability to infect important turfgrasses, such as Kentucky bluegrass, which do not naturally form associations with the fungal endophytes. We are studying the host-endophyte interaction in fine fescue (Festuca rubra). We previously developed a set of endophyte-free and endophyte-infected fine fescues in which obvious phenotype variations were documented among plants (Johnson-Cicalese et al. 2000). Our goal is to obtain quantitative transcriptome comparisons between endophyte-free and endophyte-infected plants. Our hypothesis is that the host plant sense, in some as yet unknown way, the presence of the fungal endophyte and its transcriptome is altered in ways that are important to maintenance of the symbiotic interaction. Our approach is to use SOLiD-SAGE to compare the transcriptomes of endophyte-free and endophyte-infected fine-fescue. With the SOLiD-SAGE method we can obtain a quantitative assessment of the plant and fungal genes that are expressed. We will be comparing the results from endophyte-infected and endophyte-free plants of the identical genotype. Because of the massive sequencing capability of the SOLiD system, we expect 10-15 million sequences from each sample, which will give an unprecedented amount of information on the genes expressed by the host and the endophyte. Differences between the samples in the plant genes will reveal which genes are affected by the presence of the fungal endophyte. We will also obtain quantitative data on the fungal genes that are expressed in the interaction. This information will be critical to understanding the basis of compatible interactions." |
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| Language: | English |
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| References: | 1 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ambrose, K., R. A. Kerstetter, J. F. White, and F. C. Belanger. 2010. Deep transcriptome comparison of endophyte-free and endophyte-infected fine fescue. Proc. Annu. Rutgers Turfgrass Symp. p. 36. |
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| Web URL(s):
https://turf.rutgers.edu/research/abstracts/symposium2010.pdf#page=36
Last checked: 02/06/2017
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b3696858 |
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