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| DOI: | 10.1111/j.1439-0523.2006.01193.x |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.2006.01193.x/full
Last checked: 01/16/2014
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.2006.01193.x/pdf
Last checked: 01/16/2014
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Access conditions: Item is within a limited-access website |
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| Publication Type:
| Report |
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| Author(s): | Li, R.;
Bruneau, A. H.;
Qu, R. |
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| Author Affiliation: | Department of Crop Science, North Carolina State University, Raleigh, NC |
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| Title: | Improved plant regeneration and in vitro somatic embryogenesis of St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] |
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| Source: | Plant Breeding. Vol. 125, No. 1, February 2006, p. 52-56. |
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| Publishing Information: | Berlin: P. Parey |
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| # of Pages: | 5 |
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| Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.2006.01193.x/abstract
Last checked: 01/16/2014
Notes: Abstract only |
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| Keywords: | TIC Keywords: Benzyladenine; Calli; In vitro; Regeneration; Somatic embryogenesis; Stenotaphrum secundatum; Tissue culture; Tissue testing
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| Abstract/Contents: | "St Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is an important warm season turf and pasture grass. In vitro tissue culture of St Augustinegrass could serve as an important mean for its improvement through genetic transformation as well as induced somaclonal variation. To optimize tissue culture conditions for plant regeneration of St Augustinegrass, tissue culture responses of 11 explant tissues and four callus induction/subculture media have been examined. Embryogenic calli with regeneration potential were observed on cultures of early immature embryo [3 days after pollination (DAP)], immature embryo (7-14 DAP), and shoot base of young seedlings. The addition of benzyladenine (BA) in the callus induction/subculture medium enhances callus regeneration ability and does not harm callus induction for immature embryos. The best response came from 7 to 14 DAP immature embryo on MS medium containing 1 mg/l 2,4-dichlorophenoxyacetic acid and 0.5 mg/l BA. The callus induction and regeneration rates were 97.7% and 47.6% respectively. However, BA supplement reduced callus formation and failed to enhance regeneration for young leaf bases. Scanning electron microscopy revealed that plant regeneration of St Augustinegrass is via somatic embryogenesis." |
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| Language: | English |
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| References: | 19 |
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| Note: | Pictures, color
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Li, R., and A. H. Bruneau. 2006. Improved plant regeneration and in vitro somatic embryogenesis of St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze]. Plant Breed. 125(1):p. 52-56. |
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| DOI: 10.1111/j.1439-0523.2006.01193.x |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.2006.01193.x/full
Last checked: 01/16/2014
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.2006.01193.x/pdf
Last checked: 01/16/2014
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: SB 119 .A1 Z4 |
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