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| DOI: | 10.21273/HORTSCI.44.5.1254 |
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| Web URL(s): | https://journals.ashs.org/hortsci/view/journals/hortsci/44/5/article-p1254.xml
Last checked: 12/06/2019
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Sato, Hiroko;
Takamizo, Tadashi;
Shimizu, Tsutomu;
Kawai, Kiyoshi;
Kaku, Koichiro |
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| Author Affiliation: | Sato and Takamizo: Forage Crop Biotechnology Research Team, National Institute of Livestock and Grassland Science, Nasushiobara, Tochigi, Japan; Shimizu, Kawai and Kaku: Life Science Research Institute, Kumiai Chemical Industry Co., Shizuoka, Japan |
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| Title: | Conferred resistance to an acetolactate synthase-inhibiting herbicide in transgenic tall fescue (Festuca arundinacea Schreb.) |
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| Section: | Breeding, cultivars, rootstocks, and germplasm resources
Other records with the "Breeding, cultivars, rootstocks, and germplasm resources" Section
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| Source: | HortScience. Vol. 44, No. 5, August 2009, p. 1254-1257. |
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| Publishing Information: | Alexandria, VA: American Society for Horticultural Science |
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| # of Pages: | 4 |
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| Keywords: | TIC Keywords: Acetolactate synthase; Acetolactate synthase-inhibiting herbicides; Festuca arundinacea; Herbicide resistance; Transgenic turfgrasses
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| Abstract/Contents: | "Herbicide-resistant turfgrass can be an efficient tool that will allow easier turf maintenance. Acetolactate synthase (ALS) is the first common enzyme in the biosynthetic pathways leading to the branched-chain amino acids, and amino acid substitutions in ALS have been known to confer resistance to ALS-inhibiting herbicides. A two-point mutated rice ALS gene [OsALS (dm)] has been shown to confer strong resistance to bispyribac-sodium (BS), an ALS-inhibiting herbicide. In this study, we introduced into turf-type tall fescue (Festuca arundinacea Schreb.) the OsALS (dm) gene by using Agrobacterium-mediated transformation for conferring herbicide resistance. Stable integration of the transgene was confirmed by Southern blot analysis. Transgenic and wild-type plants were sprayed on the leaves with herbicide containing BS; approximately half of the transgenic plants were unaffected by the treatment and showed resistance to the herbicide, whereas the wild-type plants died. ALS activity in the leaf tissue of transgenic-resistant plants incubated with BS was almost equivalent to that in wild-type plants without BS and was higher than in wild-type plants incubated with BS. These indicate that the transgenic-resistant plants actively produced OsALS (dm) protein under herbicide treatment. This is the first report of herbicide-resistant transgenic tall fescue after introduction of a mutated ALS gene." |
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| Language: | English |
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| References: | 14 |
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| Note: | Pictures, color
Figures
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sato, H., T. Takamizo, T. Shimizu, K. Kawai, and K. Kaku. 2009. Conferred resistance to an acetolactate synthase-inhibiting herbicide in transgenic tall fescue (Festuca arundinacea Schreb.). HortScience. 44(5):p. 1254-1257. |
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| DOI: 10.21273/HORTSCI.44.5.1254 |
| Web URL(s):
https://journals.ashs.org/hortsci/view/journals/hortsci/44/5/article-p1254.xml
Last checked: 12/06/2019
Requires: PDF Reader |
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MSU catalog number: b2217685a |
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