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| DOI: | 10.21273/JASHS.135.1.74 |
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| Web URL(s): | https://journals.ashs.org/jashs/view/journals/jashs/135/1/article-p74.xml
Last checked: 12/06/2019
Requires: PDF Reader |
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| Publication Type:
| Refereed |
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| Author(s): | Harris, Karen R.;
Schwartz, Brian M.;
Paterson, Andrew H.;
Brady, Jeff A. |
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| Author Affiliation: | Harris: U.S. Department of Agriculture-Agriculture Research Service, Crop Genetics and Breeding Research; Schwartz: Department of Crop and Soil Sciences, University of Georgia, Tifton Campus, Tifton; Paterson: Plant Genome Mapping Laboratory, University of Georgia, Athens, GA; Brady: Texas AgriLife Research, Stephenville, TX |
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| Title: | Identification and mapping of nucleotide binding site-leucine-rich repeat resistance gene analogs in bermudagrass |
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| Section: | Molecular biology-biotechnology
Other records with the "Molecular biology-biotechnology" Section
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| Source: | Journal of The American Society for Horticultural Science. Vol. 135, No. 1, January 2010, p. 74-82. |
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| Publishing Information: | Alexandria, Va.: American Society for Horticultural Science |
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| # of Pages: | 9 |
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| Keywords: | TIC Keywords: Cynodon; Disease resistance; Gene mapping; Genetic characterization; Genetics
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| Abstract/Contents: | "Thirty-one partial bermudagrass (Cynodon spp.) disease-resistance gene analogs (BRGA) were cloned and sequenced from diploid, triploid, tetraploid, and hexaploid bermudagrass using degenerate primers to target the nucleotide binding site (NBS) of the NBS-leucine-rich repeat (LRR) resistance gene family. Alignment of deduced amino acid sequences revealed that the conserved motifs of the NBS are present and all sequences have non-Drosophila melanogaster Toll and mammalian interleukin-1 receptor (TIR) motifs. Using a neighbor-joining algorithm, a dendrogram was created and nine groups of deduced amino acid sequences from bermudagrass could be identified from those sequences that span the NBS. Four BRGA markers and 15 bermudagrass expressed sequence tags (ESTs) with similarity to resistance genes or resistance gene analogs were placed on a bermudagrass genetic map. Multiple BRGA and EST markers mapped on T89 linkage groups 1a and 5a and clusters were seen on T89 19 and two linkage groups previously unidentified. In addition, three primers made from BRGA groups and ESTs with similarity to NBS-LRR resistance genes amplify NBS-LRR analogs in zoysiagrass (Zoysia japonica or Z. matrella) or seashore paspalum (Paspalum vaginatum). This gives evidence of conservation of NBS-LRR analogs among the subfamilies Chloridoideae and Panicoideae. Once disease resistance genes are identified, these BRGA and EST markers may be useful in marker-assisted selection for the improvement of disease resistance in bermudagrass." |
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| Language: | English |
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| References: | 57 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Harris, K. R., B. M. Schwartz, A. H. Paterson, and J. A. Brady. 2010. Identification and mapping of nucleotide binding site-leucine-rich repeat resistance gene analogs in bermudagrass. J. Am. Soc. Hortic. Sci. 135(1):p. 74-82. |
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| DOI: 10.21273/JASHS.135.1.74 |
| Web URL(s):
https://journals.ashs.org/jashs/view/journals/jashs/135/1/article-p74.xml
Last checked: 12/06/2019
Requires: PDF Reader |
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MSU catalog number: b2221587a |
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