Full TGIF Record # 162488
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DOI:10.21273/JASHS.135.2.108
Web URL(s):https://journals.ashs.org/jashs/view/journals/jashs/135/2/article-p108.xml
    Last checked: 12/06/2019
    Requires: PDF Reader
Publication Type:
i
Refereed
Author(s):Zhang, Yan; Liang, Cuiyue; Xu, Yan; Gianfagna, Thomas; Huang, Bingru
Author Affiliation:Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ
Title:Effects of ipt gene expression on leaf senescence induced by nitrogen or phosphorus deficiency in creeping bentgrass
Section:Environmental stress physiology
Other records with the "Environmental stress physiology" Section
Source:Journal of The American Society for Horticultural Science. Vol. 135, No. 2, March 2010, p. 108-115.
Publishing Information:Alexandria, Va.: American Society for Horticultural Science
# of Pages:8
Keywords:TIC Keywords: Agrostis stolonifera; Chlorophyll; Cytokinins; Gene expression; Nitrogen; Nutrient deficiency; Phosphorus; Senescence
Abstract/Contents:"The objective of the study was to determine whether the expression of a cytokinin (CK) biosynthesis gene encoding adenine isopentenyl transferase (ipt) would delay or suppress leaf senescence induced by nitrogen (N) or phosphorus (P) deficiency in a C3 grass species, creeping bentgrass (Agrostis stolonifera). The ipt gene was ligated to a senescence-associated promoter, SAG12, and was transferred into creeping bentgrass using an agrobacterium (Agrobacterium tumefaciens)-mediated transformation technique. Plants from an SAG12-ipt transgenic line (S41) and a null transformant (NT) control line were grown in nutrient solutions with all essential elements or without N (-N) or P (-P) for 21 days. Significant declines in leaf photochemical efficiency (Fv/Fm) and chlorophyll content of mature leaves were detected in NT and SAG12-ipt plants exposed to N or P deficiency. Compared to the NT control line, SAG12-ipt plants had higher levels of Fv/Fm, chlorophyll, and CK contents in leaves, and these differences between the NT control and SAG12-ipt line became more pronounced with treatment duration. The ipt expression was detected in the -P-treated and the -N-treated plants after 21 days, although the level of expression decreased under N or P deficiency. Under -P treatment, root acid phosphatase activity was greater in SAG12-ipt line than in the NT control line. No significant differences in nitrate reductase activity were detected in leaves or roots between the SAG12-ipt and the NT control lines. Our results demonstrated that SAG12-ipt expression suppressed leaf senescence induced by N or P deficiency in a perennial grass species. The suppressing effects on leaf senescence under P deficiency may be related to CK regulation of more efficient use of P in roots of the SAG12-ipt plants."
Language:English
References:Unknown
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Zhang, Y., C. Liang, Y. Xu, T. Gianfagna, and B. Huang. 2010. Effects of ipt gene expression on leaf senescence induced by nitrogen or phosphorus deficiency in creeping bentgrass. J. Am. Soc. Hortic. Sci. 135(2):p. 108-115.
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DOI: 10.21273/JASHS.135.2.108
Web URL(s):
https://journals.ashs.org/jashs/view/journals/jashs/135/2/article-p108.xml
    Last checked: 12/06/2019
    Requires: PDF Reader
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