Full TGIF Record # 162860
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DOI:10.1007/s11104-009-0241-5
Web URL(s):https://link.springer.com/article/10.1007/s11104-009-0241-5
    Last checked: 10/04/2017
Publication Type:
i
Refereed
Author(s):Riley, Ian T.; Wiebkin, Sue; Hartley, Diana; McKay, Alan C.
Author Affiliation:Riley, Wiebkin and McKay: Plant and Soil Health, SARDI, Plant Research Centre, Hartley Grove, Urrbrae; Riley: School of Agricultural Food and Wine, University of Adelaide, Adelaide, SA; Hartley: CSIRO Plant Industry, Canberra, ACT, Australia
Title:Quantification of roots and seeds in soil with real-time PCR
Source:Plant and Soil. Vol. 331, No. 1-2, June 2010, p. 151-163.
# of Pages:13
Publishing Information:Dordrecht, Netherlands: Kluwer Academic Publishers
Keywords:TIC Keywords: DNA; Dry weight; Herbicide application; Lolium perenne; Polymerase chain reaction; Roots; Soils; Trifolium subterraneum
Abstract/Contents:"Study of roots and associated organisms in soil particularly in mixed plant populations, such as pastures, is limited by difficulties in quantification of root growth and function. The research evaluated the potential of DNA quantification by real-time PCR to improve our capacity to study and understand roots in such contexts. Probes and primers were developed for two common pasture species, Trifolium subterraneum and Lolium perenne (and closely related lolium spp.), and evaluated for specificity and sensitivity in TaqMan assays on DNA extracted from soil. Further experiments examined the ability to detect DNA in dead roots, the changes in root DNA levels of plants defoliated or treated with herbicide and the relationship between DNA and root dry weight for single and mixed plant species grown in pots. T. Subterraneum DNA/PCR 200 fg/μl was detected at 17.5 cycles and L. perenne at 19.5 cycles. The assay for T. Subterraneum was species specific but the L. perenne assay, as anticipated from the choice of probe, also detected some closely related species. The assays were sensitive and capable of detecting equivalent to <2 mg roots/kg of dry soil and able to quantify targets in mixed populations. DNA concentration varied with plant age and genotype and DNA in dead roots found to decay rapidly over a few days. DNA concentrations in roots were found to respond more rapidly to defoliation and herbicide treatments than root mass. This approach appears to offer a new way to study roots in soil and indicates that quantifying root DNA could provide insights into root function and responses not readily provided by other methods."
Language:English
References:18
Note:Figures
Tables
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Riley, I. T., S. Wiebkin, D. Hartley, and A. C. McKay. 2010. Quantification of roots and seeds in soil with real-time PCR. Plant Soil. 331(1-2):p. 151-163.
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DOI: 10.1007/s11104-009-0241-5
Web URL(s):
https://link.springer.com/article/10.1007/s11104-009-0241-5
    Last checked: 10/04/2017
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MSU catalog number: b2212822
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