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DOI: | 10.1111/j.14390434.2009.01660.x |
Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/full Last checked: 01/31/2014 Access conditions: Item is within a limited-access website http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/pdf Last checked: 06/09/2010 Requires: PDF Reader Access conditions: Item is within a limited-access website |
Publication Type:
| Refereed |
Author(s): | Asano, Takahiro;
Senda, Masako;
Suga, Haruhisa;
Kageyama, Koji |
Author Affiliation: | Asano, Senda, and Kageyama: River Basin Research Center; Suga: Life Science Research Center, Gifu University, Gifu, Japan |
Title: | Development of multipex PCR to detect five Pythium species related to turfgrass diseases |
Source: | Journal of Phytopathology. Vol. 158, No. 1, January 2010, p. 1-7. |
Publishing Information: | Berlin: Blackwell Wissenschafts-Verlag |
# of Pages: | 7 |
Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/abstract Last checked: 01/31/2014 Notes: Abstract only |
Keywords: | TIC Keywords: DNA amplification; Disease identification; Gene mapping; Multiplex polymerase chain reaction; Pythium aphanidermatum; Pythium arrhenomanes; Pythium graminicola; Pythium torulosum; Pythium vanterpoolii
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Abstract/Contents: | "The objective of this study was to develop multiplex PCR detection method for five Pythium species associated with turfgrass diseases, Pythium aphanidermatum, Pythium arrhenomanes, Pythium graminicola, Pythium torulosum and Pythium vanterpoolii. Species-specific primers and two common primers were designed based on the sequences of the internal transcribed spacer region of ribosomal DNA. Another primer set by which all organisms would be amplified in 18S rDNA was used as a positive control. When these total nine primers were applied to the multiplex PCR, all species were individually discriminated in the mixture of five species culture DNA. Furthermore, all five Pythium species were detected in naturally infected plants using the multiplex PCR." |
Language: | English |
References: | 23 |
Note: | Reprint appears in Journal of Phytopathology, 158(9) September 2010, p. 609-615 Figures Tables |
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete): Asano, T., M. Senda, and K. Kageyama. 2010. Development of multipex PCR to detect five Pythium species related to turfgrass diseases. J. Phytopathol. 158(1):p. 1-7. |
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| DOI: 10.1111/j.14390434.2009.01660.x |
| Web URL(s): http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/full Last checked: 01/31/2014 Access conditions: Item is within a limited-access website http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/pdf Last checked: 06/09/2010 Requires: PDF Reader Access conditions: Item is within a limited-access website |
| MSU catalog number: b2219735 |
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