Full TGIF Record # 163143
Item 1 of 1
DOI:10.1111/j.14390434.2009.01660.x
Web URL(s):http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/full
    Last checked: 01/31/2014
    Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/pdf
    Last checked: 06/09/2010
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Publication Type:
i
Refereed
Author(s):Asano, Takahiro; Senda, Masako; Suga, Haruhisa; Kageyama, Koji
Author Affiliation:Asano, Senda, and Kageyama: River Basin Research Center; Suga: Life Science Research Center, Gifu University, Gifu, Japan
Title:Development of multipex PCR to detect five Pythium species related to turfgrass diseases
Source:Journal of Phytopathology. Vol. 158, No. 1, January 2010, p. 1-7.
Publishing Information:Berlin: Blackwell Wissenschafts-Verlag
# of Pages:7
Related Web URL:http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/abstract
    Last checked: 01/31/2014
    Notes: Abstract only
Keywords:TIC Keywords: DNA amplification; Disease identification; Gene mapping; Multiplex polymerase chain reaction; Pythium aphanidermatum; Pythium arrhenomanes; Pythium graminicola; Pythium torulosum; Pythium vanterpoolii
Abstract/Contents:"The objective of this study was to develop multiplex PCR detection method for five Pythium species associated with turfgrass diseases, Pythium aphanidermatum, Pythium arrhenomanes, Pythium graminicola, Pythium torulosum and Pythium vanterpoolii. Species-specific primers and two common primers were designed based on the sequences of the internal transcribed spacer region of ribosomal DNA. Another primer set by which all organisms would be amplified in 18S rDNA was used as a positive control. When these total nine primers were applied to the multiplex PCR, all species were individually discriminated in the mixture of five species culture DNA. Furthermore, all five Pythium species were detected in naturally infected plants using the multiplex PCR."
Language:English
References:23
Note:Reprint appears in Journal of Phytopathology, 158(9) September 2010, p. 609-615
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Asano, T., M. Senda, and K. Kageyama. 2010. Development of multipex PCR to detect five Pythium species related to turfgrass diseases. J. Phytopathol. 158(1):p. 1-7.
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DOI: 10.1111/j.14390434.2009.01660.x
Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/full
    Last checked: 01/31/2014
    Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/pdf
    Last checked: 06/09/2010
    Requires: PDF Reader
    Access conditions: Item is within a limited-access website
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