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| DOI: | 10.1111/j.1439-0434.2009.01660.x |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/pdf
Last checked: 08/23/2010
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http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/full
Last checked: 08/23/2010
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | Asano, Takahiro;
Senda, Masako;
Suga, Haruhisa;
Kageyama,Koji |
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| Author Affiliation: | Asano: Geological Isolation Research and Development Directorate, Japan Atomic Energy Agency, Muramatsu Tokai-Mura, Naka-Gun, Ibaraki; Senda and Kageyama: River Basin Research Center; Suga: Life Science Research Center, Gifu University, Yanagido, Gifu, Japan; |
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| Title: | Development of multiplex PCR to detect five Pythium species related to turfgrass diseases |
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| Source: | Journal of Phytopathology. Vol. 158, No. 9, September 2010, p. 609-615. |
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| Publishing Information: | Berlin: Blackwell Wissenschafts-Verlag |
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| # of Pages: | 7 |
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| Keywords: | TIC Keywords: DNA amplification; Differentiation; Disease identification; Genetic markers; Polymerase chain reaction; Hosts of plant pests; Isolation technique; Pythium diseases
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| Abstract/Contents: | "The objective of this study was to develop multiplex PCR detection method for five pythium species associated with turfgrass diseases, Pythium aphanidermatum, Pythium arrhenomanes, Pythium graminicola, Pythium torulosum and Pythium vanterpoolii. Species-specific primers and two common primers were designed based on the sequences of the internal transcribed spacer region of ribosomal DNA. Another primer set by which all organisms would be amplified in 18S rDNA was used as a positive control. When these total nine primers were applied to the multiplex PCR, all species were individually discriminated in the mixture of five species culture DNA. Furthermore, all five pythium species were detected in naturally infected plants using the multiplex PCR." |
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| Language: | English |
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| References: | 23 |
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| Note: | Figures
Tables |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Asano, T., M. Senda, H. Suga, and K. Kageyama. 2010. Development of multiplex PCR to detect five Pythium species related to turfgrass diseases. J. Phytopathol. 158(9):p. 609-615. |
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| DOI: 10.1111/j.1439-0434.2009.01660.x |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/pdf
Last checked: 08/23/2010
Requires: PDF Reader
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2009.01660.x/full
Last checked: 08/23/2010
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2219735 |
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