| |
| DOI: | 10.1007/s00122-010-1360-3 |
|---|
| Web URL(s): | https://link.springer.com/article/10.1007%2Fs00122-010-1360-3
Last checked: 10/04/2017 |
|---|
| Publication Type:
| Refereed |
|---|
| Author(s): | Sandhu, Sukhpreet;
Blount, Ann R.;
Quesenberry, Kenneth H.;
Altpeter, Fredy |
|---|
| Author Affiliation: | Sandhu, Quesenberry and Altpeter: Plant Molecular and Cellular Biology Program, Agronomy Department, Genetics Institute, University of Florida, IFAS, Gainesville; Blount: North Florida Research and Education Center, University of Florida, IFAS, Marianna, FL, USA; Sandhu: Center for Applied Genetic Technologies, Institute of Plant Breeding Genetics and Genomics, University of Georgia, Athens, GA, USA |
|---|
| Title: | Apomixis and ploidy barrier suppress pollen-mediated gene flow in field grown transgenic turf and forage grass (Paspalum notatum Flüggé) |
|---|
| Section: | Original paper
Other records with the "Original paper" Section
|
|---|
| Source: | TAG: Theoretical and Applied Genetics. Vol. 121, No. 5, September 2010, p. 919-929. |
|---|
| Publishing Information: | Berlin: Springer |
|---|
| # of Pages: | 11 |
|---|
| Related Web URL: | http://www.springerlink.com/content/258n257677335561/MediaObjects/122_2010_1360_MOESM1_ESM.doc
Last checked: 08/25/2010
Requires: Microsoft Word
Access conditions: Document is within a limited-access website
Notes: Recordings During the Highest Transgenic Seed Head Production |
|---|
| Keywords: | TIC Keywords: Apomixis; Diploids; Genetic transformation; Herbicide resistance; Hybridization; Paspalum notatum; Seed production; Transgenes
|
|---|
| Cultivar Names: | Argentine |
|---|
| Abstract/Contents: | "Bahiagrass (Paspalum notatum Flüggé) is the predominant forage grass in the southeastern US. The commercially important bahiagrass cultivar 'Argentine' is preferred for genetic transformation over sexual diploid cytotypes, since it produces uniform seed progeny through apomixis. Pseudogamous apomictic seed production in Argentine bahiagrass may contribute to transgene confinement. It is characterized by embryo development which is independent of fertilization of the egg cell, but requires fertilization with compatible pollen to produce the endosperm. Pollen-mediated gene transfer from transgenic, glufosinate-resistant apomictic bahiagrass as pollen donor at close proximity (0.5-3.5 m) with non-transgenic sexual or apomictic bahiagrass cultivars as pollen receptors was evaluated under field conditions. Hybridization frequency was evaluated by glufosinate herbicide resistance in >23,300 seedlings derived from open-pollinated (OP) pollen receptor plants. Average gene transfer between transgenic apomictic, tetraploid and sexual diploid bahiagrass was 0.03%. Herbicide-resistant hybrids confirmed by immuno-chromatographic detection of the PAT protein displayed a single copy bar gene identical to the pollen parent. Hybrids resulting from diploid pollen receptors were confirmed as triploids or aneu-triploids with significantly reduced vigor and seed set as compared to the parents. Transmission of transgenes to sexual bahiagrass is severely restricted by the ploidy difference between tetraploid apomicts and diploid sexual bahiagrass. Average gene transfer between transgenic apomictic tetraploid and non-transgenic, apomictic tetraploid bahiagrass was 0.17%, confirming a very low frequency of amphimixis in apomictic bahiagrass cultivars. While not providing complete transgene containment, gene transfer between transgenic apomictic and non-transgenic bahiagrass occurs at a much lower frequency than reported for other cross-pollinating or facultative apomictic grasses." |
|---|
| Language: | English |
|---|
| References: | 58 |
|---|
| Note: | Pictures, color
Figures
Tables
Graphs |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Sandhu, S., A. R. Blount, K. H. Quesenberry, and F. Altpeter. 2010. Apomixis and ploidy barrier suppress pollen-mediated gene flow in field grown transgenic turf and forage grass (Paspalum notatum Flüggé). Theor. Appl. Genet. 121(5):p. 919-929. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=168008 |
| If there are problems with this record, send us feedback about record 168008. |
| Choices for finding the above item: |
|---|
| DOI: 10.1007/s00122-010-1360-3 |
| Web URL(s):
https://link.springer.com/article/10.1007%2Fs00122-010-1360-3
Last checked: 10/04/2017 |
| 
MSU catalog number: b2203373 |
| Find from within TIC:
Digitally in TIC by record number. |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
