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| DOI: | 10.1111/j.1439-0434.2010.01717.x |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2010.01717.x/full
Last checked: 09/07/2010
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2010.01717.x/pdf
Last checked: 09/07/2010
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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| Publication Type:
| Refereed |
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| Author(s): | De Weerdt, Marjanne;
Kox, Linda;
Waeyenberge, Lieven;
Viaene, Nicole;
Zijlstra, Carolien |
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| Author Affiliation: | Weerdt and Zijlstra: Plant Research International; Kox: Plantenziektenkundige Dienst, Wageningen, Netherlands; Waeyenberger and Viaene: Institute for Agricultural and Fisheries Research, Merelbeke, Belgium |
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| Title: | A real-time PCR assay to identify Meloidogyne minor |
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| Source: | Journal of Phytopathology. Vol. 159, No. 2, February 2011, p. 80-84. |
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| Publishing Information: | Berlin: Blackwell Wissenschafts-Verlag |
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| # of Pages: | 5 |
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| Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2010.01717.x/abstract
Last checked: 09/07/2010
Notes: Abstract only |
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| Keywords: | TIC Keywords: Characteristics; DNA amplification; Identification; Injuries by insects; Isoenzyme analysis; Isolation technique; Meloidogyne minor; Methodology; Nematoda; Polymerase chain reaction; Rhizoctonia yellow patch; Symptoms
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| Abstract/Contents: | "Meloidogyne minor is a small root-knot nematode that causes yellow patch disease in golf courses and severe quality damage in potatoes. It was described in 2004 and has been detected in The Netherlands, England, Wales, Northern Ireland, Ireland and Belgium. The nematode often appears together with M. naasi on grasses. It causes similar symptoms on potato tubers as M. chitwoodi and M. fallax, which are both quarantine organisms in Europe. An accurate identification method therefore is required. This study describes a real-time PCR assay that enables the identification of M. minor after extraction of nematodes from soil or plant samples. Alignments of sequences of rDNA-ITS fragments of M. minor and five other Meloidogyne species were used to design a forward primer Mminor f299, a specific primer Mminor r362 and the specific MGB TaqMan probe P Mm MGB321. PCR with this primers and probe results in an amplicon of 64 bp. The analytical specificity of the real-time PCR assay was assessed by assaying it on six populations of M. minor and on 10 populations of six other Meloidogyne species. Only DNA from M. minor gave positive results in this assay. The assay was able to identify M. minor using DNA from a single juvenile independent from the DNA extraction method used." |
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| Language: | English |
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| References: | 18 |
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| Note: | Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
De Weerdt, M., L. Kox, L. Waeyenberge, N. Viaene, and C. Zijlstra. 2011. A real-time PCR assay to identify Meloidogyne minor. J. Phytopathol. 159(2):p. 80-84. |
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| DOI: 10.1111/j.1439-0434.2010.01717.x |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2010.01717.x/full
Last checked: 09/07/2010
Access conditions: Item is within a limited-access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2010.01717.x/pdf
Last checked: 09/07/2010
Requires: PDF Reader
Access conditions: Item is within a limited-access website |
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MSU catalog number: b2219735 |
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