| |
| DOI: | 10.1094/PHYTO.2010.100.6.S1 |
|---|
| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2010.100.6.S1#page=75
Last checked: 11/22/2010
Requires: PDF Reader |
|---|
| Publication Type:
| Report |
|---|
| Content Type: | Abstract or Summary only |
|---|
| Author(s): | Ma, B.;
Tredway, L. P. |
|---|
| Author Affiliation: | North Carolina State University, Raleigh, NC |
|---|
| Title: | Demethylation inhibitor (DMI) fungicide resistance mechanism in Sclerotinia homeocarpa causing dollar spot in turfgrasses |
|---|
| Section: | 2010 APS Annual Meeting abstracts of presentations
Other records with the "2010 APS Annual Meeting abstracts of presentations" Section
|
|---|
| Source: | Phytopathology. Vol. 100, No. 6S, June 2010, p. S75. |
|---|
| Publishing Information: | St. Paul, MN: American Phytopathological Society |
|---|
| # of Pages: | 1 |
|---|
| Keywords: | TIC Keywords: Clones; DMI resistance; Dollar spot; Gene expression; Genetic engineering; Sclerotinia homoeocarpa
|
|---|
| Abstract/Contents: | "The cytochrome P450 sterol 14α-demethylase gene (ShCYP51) in Sclerotinia homoeocarpa was cloned and sequenced. ShCYP51 gene was 1680 bp in length and contained two introns of 53 bp and 57 bp located after nucleotide positions in 247 and 498, respectively. The deduced amino acid sequence had a similarity of 73.0, 70.5 and 54.5% to the CYP51 genes from Monilinia fructicola, Botryotinia fuckeliana, and Venturia inaequalis, respectively. An ~1700 bp fragment in the upstream region of the gene was also amplified and sequenced. It was predicted to have two promoters located at the base pair position of -122 to -172 and -791 to -841 of the gene, respectively. The sensitivities of 43 S. homoeocarpa isolates to DMI fungicide were determined in vitro using a discriminatory dose of 0.02 μg/ml of propiconazole. The ShCYP51 genes and the promoter regions from those isolates were sequenced and compared. Three types of point mutation were detected among 12 isolates; however, none of them were associated with DMI sensitivity. No inserts or repeats was found in the promoter region from any of the isolates. Real-time PCR was used to quantify ShCYP51 gene expression in six sensitive and six resistant isolates, and no difference was found between the two types of isolates. Results from this study show that point mutation and over-expression of the ShCYP51 gene and modifications of the genes promoter region are not the mechanisms operating in S. homoeocarpa leading to the resistance to DMI fungicide." |
|---|
| Language: | English |
|---|
| References: | 0 |
|---|
| Note: | This item is an abstract only! |
|---|
| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Ma, B., and L. P. Tredway. 2010. Demethylation inhibitor (DMI) fungicide resistance mechanism in Sclerotinia homeocarpa causing dollar spot in turfgrasses. Phytopathology. 100(6S):p. S75. |
| Fastlink to access this record outside TGIF: https://tic.msu.edu/tgif/flink?recno=172715 |
| If there are problems with this record, send us feedback about record 172715. |
| Choices for finding the above item: |
|---|
| DOI: 10.1094/PHYTO.2010.100.6.S1 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2010.100.6.S1#page=75
Last checked: 11/22/2010
Requires: PDF Reader |
| 
MSU catalog number: b2219736a |
| Find from within TIC:
Digitally in TIC by file name: phytp2010junpres |
| Request through your local library's inter-library loan service (bring or send a copy of this TGIF record) |
