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| DOI: | 10.1094/PHYTO.2010.100.6.S1 |
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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2010.100.6.S1#page=64
Last checked: 11/23/2010
Requires: PDF Reader
Notes: Item is within a single large file |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Koch, P.;
Kerns, J. |
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| Author Affiliation: | Koch: University of Wisconsin, Verona, WI; Kerns: University of Wisconsin, Madison, WI |
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| Title: | Validation of commercially available ELISA kits for analyzing chlorothalonil and iprodione residues on creeping bentrgrass using gas chromatography |
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| Section: | 2010 APS Annual Meeting abstracts of presentations
Other records with the "2010 APS Annual Meeting abstracts of presentations" Section
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| Source: | Phytopathology. Vol. 100, No. 6S, June 2010, p. S64. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Agrostis stolonifera; Chlorothalonil; Comparisons; Enzyme-linked immunosorbent assay; Fungicides; Gas chromatography; Iprodione
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| Abstract/Contents: | "Commercially available enzyme-linked immunosorbent assay (ELISA) kits (Horiba, Ltd) can determine concentrations of chlorothalonil and iprodione on fruits and grains quickly and accurately. To be used on turfgrasses, modifications to the ELISA protocol provided by Horiba, Ltd were required to accommodate smaller quantities of plant material collected from creeping bentgrass mowed at fairway height. The amount of tissue was reduced from 5 g to 0.2 g and the tissue was pulverized with a solvent for extraction of the fungicides with/within the plant tissue. Validation of the modified ELISA method was conducted in the fall of 2009 by comparing fungicide concentrations collected from ELISA and gas chromatography/flame ionized detection (GC/FID). This was accomplished by analyzing six samples of creeping bentgrass for fungicide concentration one hour following the application of chlorothalonil and iprodione using the altered ELISA method as well as GC/FID. Chlorothalonil was not accurately detected using the ELISA method, while measured levels of iprodione were consistently five times lower than what was detected with GC/FID. The ELISA method was further modified to be more consistent with the Horiba, Ltd protocol, and subsequent comparisons between ELISA and GC/FID were completed on April 14 and May 12. A reliable ELISA method for the detection of fungicides in turfgrass can be used to provide insight into the role that fungicides play in disease control." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Koch, P., and J. Kerns. 2010. Validation of commercially available ELISA kits for analyzing chlorothalonil and iprodione residues on creeping bentrgrass using gas chromatography. Phytopathology. 100(6S):p. S64. |
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| DOI: 10.1094/PHYTO.2010.100.6.S1 |
| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2010.100.6.S1#page=64
Last checked: 11/23/2010
Requires: PDF Reader
Notes: Item is within a single large file |
| 
MSU catalog number: b2219736a |
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Digitally in TIC by file name: phytp2010junpres |
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