Full TGIF Record # 172992
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DOI:10.21273/JASHS.135.6.511
Web URL(s):https://journals.ashs.org/jashs/view/journals/jashs/135/6/article-p511.xml
    Last checked: 04/30/2020
https://journals.ashs.org/jashs/downloadpdf/journals/jashs/135/6/article-p511.xml
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Publication Type:
i
Refereed
Author(s):Harris-Shultz, Karen R.; Schwartz, Brian M.; Hanna, Wayne W.; Brady, Jeff A.
Author Affiliation:Harris-Shultz: USDA-ARS, Crop Genetics and Breeding Research Unit; Schwartz and Hanna: Department of Crop and Soil Sciences, University of Georgia, Tifton Campus, Tifton, GA; Brady: Texas AgriLife Research, Stephenville, TX
Title:Development, linkage mapping, and use of microsatellites in bermudagrass
Section:Genetics and breeding
Other records with the "Genetics and breeding" Section
Source:Journal of The American Society for Horticultural Science. Vol. 135, No. 6, November 2010, p. 511-520.
Publishing Information:Alexandria, Va.: American Society for Horticultural Science
# of Pages:10
Keywords:TIC Keywords: Amplification fragment length polymorphisms; Cynodon dactylon; DNA amplification; Gene mapping; Genetic characterization; Cross breeding; Mutations; Simple sequence repeats
Abstract/Contents:"Genetic linkage maps of bermudagrass (Cynodon spp.) species using 118 triploid individuals derived from a cross of T89 [C. dactylon (2n = 4x = 36)] and T574 [C. transvaalensis (2n = 2x = 18)] were enriched with expressed sequence tags-derived simple sequence repeat (EST-SSR) markers. Primers were developed from 53 ESTs containing SSRs producing 75 segregating markers from which 28 could be mapped to the T89 and T574 genetic maps. With the addition of previously generated marker data, 26 T89 linkage groups and eight T574 linkage groups were formed using a log-of-odds (LOD) value of 4.0. The T89 and T574 linkage maps spanned 1055 cM and 311.1 cM and include 125 and 36 single-dose amplified fragments (SDAFs), respectively. Many of the SDAFs displayed disomic segregation and thus T89 may be a segmental allotetraploid or an allotetraploid. The additional EST-SSR markers add value to the maps by increasing marker density and provide markers that can be easily transferred to other bermudagrass populations. Furthermore, EST-SSRs can be immediately used to assess genetic diversity, identify non-mutated cultivars of bermudagrass, confirm pedigrees, and differentiate contaminants from cultivars derived from 'Tifgreen'"
Language:English
References:36
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Harris-Shultz, K. R., B. M. Schwartz, W. W. Hanna, and J. A. Brady. 2010. Development, linkage mapping, and use of microsatellites in bermudagrass. J. Am. Soc. Hortic. Sci. 135(6):p. 511-520.
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DOI: 10.21273/JASHS.135.6.511
Web URL(s):
https://journals.ashs.org/jashs/view/journals/jashs/135/6/article-p511.xml
    Last checked: 04/30/2020
https://journals.ashs.org/jashs/downloadpdf/journals/jashs/135/6/article-p511.xml
    Last checked: 04/30/2020
    Requires: PDF Reader
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