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| Web URL(s): | http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2010.100.6.S193
Last checked: 12/3/2010
Requires: PDF Reader |
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| Publication Type:
| Report |
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| Content Type: | Abstract or Summary only |
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| Author(s): | Beirn, L. A.;
Crouch, J.;
Clarke, B. B. |
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| Author Affiliation: | Beirn and Clarke: Rutgers University, New Brunswick, NJ; Crouch: USDA-ARS, Cereal Disease Laboratory, St. Paul, MN |
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| Title: | Rust diseases of cultivated turfgrasses: Understanding an old foe |
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| Section: | 2009 Northeastern Division Meeting abstracts
Other records with the "2009 Northeastern Division Meeting abstracts" Section
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| Source: | Phytopathology. Vol. 100, No. 65, June supplement 2010, p. S193. |
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| Publishing Information: | St. Paul, MN: American Phytopathological Society |
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| # of Pages: | 1 |
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| Keywords: | TIC Keywords: Cultivar susceptibility; Disease identification; Disease resistance; Genetic factors; Molecular markers; Poa pratensis; Rust
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| Abstract/Contents: | "Rust is a common disease of cool-season turfgrasses that can decrease the aesthetic and economic value of many cultivated species, particularly Kentucky bluegrass (Poa pratensis L.). Chemical control of rust is costly and sometimes ineffective; therefore the use of resistant cultivars is important for the effective management of this disease. Over the past ten years, increased susceptibility to rust has been observed for several Kentucky bluegrass cultivars in the U.S., most notably the once highly resistant 'Midnight' types. It has been theorized that new races or even new species of the pathogen may be responsible for this shift in cultivar susceptibility, but the data needed to test this hypothesis is lacking. In the current study, we are using molecular markers to evaluate turfgrass rust populations. To date, 63 rust infested leaf samples have been obtained from graminicolous hosts in North America, the United Kingdom, Australia, and Chile. A reliable DNA extraction protocol was developed and both the complete internal transcribed spacer (ITS) region and 5.8S ribosomal DNA of the samples were amplified and sequenced. Assembled sequences ranged from 682 to 701 base-pairs in length, including the partial sequences of the flanking 18S and 28S rDNA. Bayesian phylogenetic analysis identified Puccinia coronata, P. graminis, and P. striiformis from infested samples, with P. coronata and P. graminis being most prevalent. Sequence data generated from this study has been used to design species-specific molecular markers to develop a real-time PCR protocol that can be utilized by turfgrass breeders, pathologists and diagnosticians for a quick identification of turfgrass rust species." |
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| Language: | English |
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| References: | 0 |
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| Note: | This item is an abstract only! |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Beirn, L. A., J. Crouch, and B. B. Clarke. 2010. Rust diseases of cultivated turfgrasses: Understanding an old foe. Phytopathology. 100(65):p. S193. |
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| Web URL(s):
http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2010.100.6.S193
Last checked: 12/3/2010
Requires: PDF Reader |
| 
MSU catalog number: b2219736a |
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Digitally in TIC by file name: phytp2010junnortheastdiv |
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