Full TGIF Record # 173898
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DOI:10.1111/j.1744-697X.2010.00195.x
Web URL(s):http://onlinelibrary.wiley.com/doi/10.1111/j.1744-697X.2010.00195.x/pdf
    Last checked: 12/14/2010
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    Access conditions: Item is within a limited access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-697X.2010.00195.x/full
    Last checked: 12/14/2010
    Access conditions: Item is within a limited access website
Publication Type:
i
Report
Author(s):Wang, Xun; Hoshino, Yoichiro; Yamada, Toshihiko
Author Affiliation:Wang: Graduate School of Environmental Science; Hoshino and Yamada: Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, Japan
Title:Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.)
Source:Grassland Science. Vol. 56, No. 4, December 2010, p. 198-204.
# of Pages:7
Publishing Information:Oxford, England: Blackwell Pub.
Related Web URL:http://onlinelibrary.wiley.com/doi/10.1111/j.1744-697X.2010.00195.x/abstract
    Last checked: 12/14/2010
    Notes: Abstract only
Keywords:TIC Keywords: Breeding improvement; Breeding program; Calli; Embryogenesis; Forage crops; Growth rate; In vitro; Regeneration; Shoot growth; Zoysia
Abstract/Contents:"A rapid and efficient in vitro culture system has been established in zoysiagrass (Zoysia japonica Steud.). Embryos isolated from mature seeds were used to induce callus, which improved callus formation and shortened the culture period. A high concentration of 2,4-dichlorophenoxyacetic acid (5 mg L?1) in combination with low concentration of 6-benzyladenine (0.2 mg L?1) was proved to be suitable for callus induction in zoysiagrass. Eight types of calli were observed; friable callus was the most regenerable type. Shoot regeneration efficiency was improved by using 1 mg L?1 of thidiazuron with 1-naphthaleneacetic acid (0.05 mg L?1) and gibberellic acid (0.1 mg L?1). Zoysiagrass shoots continued to grow in MS medium, and rooted in half-strength MS medium. With this system, it appears the duration of in vitro regeneration could be shortened to 16 weeks for zoysiagrass, i.e. from inoculation of embryos to regeneration of plantlets with shoots and roots."
Language:English
References:66
Note:Pictures, b/w
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ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, X., Y. Hoshino, and T. Yamada. 2010. Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.). Grassland Science. 56(4):p. 198-204.
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DOI: 10.1111/j.1744-697X.2010.00195.x
Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-697X.2010.00195.x/pdf
    Last checked: 12/14/2010
    Requires: PDF Reader
    Access conditions: Item is within a limited access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-697X.2010.00195.x/full
    Last checked: 12/14/2010
    Access conditions: Item is within a limited access website
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MSU catalog number: b4979016~S1a
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