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| DOI: | 10.1111/j.1744-697X.2010.00195.x |
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| Web URL(s): | http://onlinelibrary.wiley.com/doi/10.1111/j.1744-697X.2010.00195.x/pdf
Last checked: 12/14/2010
Requires: PDF Reader
Access conditions: Item is within a limited access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-697X.2010.00195.x/full
Last checked: 12/14/2010
Access conditions: Item is within a limited access website |
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| Publication Type:
| Report |
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| Author(s): | Wang, Xun;
Hoshino, Yoichiro;
Yamada, Toshihiko |
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| Author Affiliation: | Wang: Graduate School of Environmental Science; Hoshino and Yamada: Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, Japan |
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| Title: | Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.) |
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| Source: | Grassland Science. Vol. 56, No. 4, December 2010, p. 198-204. |
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| Publishing Information: | Oxford, England: Blackwell Pub. |
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| # of Pages: | 7 |
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| Related Web URL: | http://onlinelibrary.wiley.com/doi/10.1111/j.1744-697X.2010.00195.x/abstract
Last checked: 12/14/2010
Notes: Abstract only |
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| Keywords: | TIC Keywords: Breeding improvement; Breeding program; Calli; Embryogenesis; Forage crops; Growth rate; In vitro; Regeneration; Shoot growth; Zoysia
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| Abstract/Contents: | "A rapid and efficient in vitro culture system has been established in zoysiagrass (Zoysia japonica Steud.). Embryos isolated from mature seeds were used to induce callus, which improved callus formation and shortened the culture period. A high concentration of 2,4-dichlorophenoxyacetic acid (5 mg L?1) in combination with low concentration of 6-benzyladenine (0.2 mg L?1) was proved to be suitable for callus induction in zoysiagrass. Eight types of calli were observed; friable callus was the most regenerable type. Shoot regeneration efficiency was improved by using 1 mg L?1 of thidiazuron with 1-naphthaleneacetic acid (0.05 mg L?1) and gibberellic acid (0.1 mg L?1). Zoysiagrass shoots continued to grow in MS medium, and rooted in half-strength MS medium. With this system, it appears the duration of in vitro regeneration could be shortened to 16 weeks for zoysiagrass, i.e. from inoculation of embryos to regeneration of plantlets with shoots and roots." |
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| Language: | English |
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| References: | 66 |
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| Note: | Pictures, b/w
Figures
Tables
Graphs |
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| ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Wang, X., Y. Hoshino, and T. Yamada. 2010. Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.). Grassland Science. 56(4):p. 198-204. |
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| DOI: 10.1111/j.1744-697X.2010.00195.x |
| Web URL(s):
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-697X.2010.00195.x/pdf
Last checked: 12/14/2010
Requires: PDF Reader
Access conditions: Item is within a limited access website
http://onlinelibrary.wiley.com/doi/10.1111/j.1744-697X.2010.00195.x/full
Last checked: 12/14/2010
Access conditions: Item is within a limited access website |
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MSU catalog number: b4979016 |
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