Full TGIF Record # 174907
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DOI:10.1007/s11032-010-9521-2
Web URL(s):https://link.springer.com/article/10.1007%2Fs11032-010-9521-2
    Last checked: 10/05/2017
Publication Type:
i
Refereed
Author(s):Tan, Chengcheng; Wu, Yanqi; Taliaferro, Charles M.; Anderson, Michael P.; Tauer, Chuck; Samuels, Tim
Author Affiliation:Tan, Wu, Taliaferro, Anderson, Samuels: Department of Plant and Soil Sciences; Tauer: Department of Natural Resource Ecology and Management, Oklahoma State University, Stillwater, OK
Title:Development of simple sequence repeat markers for bermudagrass from its expressed sequence tag sequences and preexisting sorghum SSR markers
Source:Molecular Breeding. Vol. 29, No. 1, January 2012, p. 23-30.
Publishing Information:The Netherlands: Kluwer Academic Publishers
# of Pages:8
Related Web URL:http://www.springerlink.com/content/1513825813877212/MediaObjects/11032_2010_9521_MOESM1_ESM.pdf
    Last checked: 01/24/2011
    Requires: Adobe Acrobat
    Access conditions: Document is within a limited-access website
    Notes: Table: Transferable sorghum SSR ID, size range and number of alleles in bermudagrass genotypes Cynodon transvaalensis 'T577', C. dactylon 'Tifton 10', and C. dactylon 'Zebra'
http://www.springerlink.com/content/1513825813877212/MediaObjects/11032_2010_9521_MOESM2_ESM.pdf
    Last checked: 01/24/2011
    Requires: Adobe Acrobat
    Access conditions: Document is within a limited-access website
    Notes: Table: Bermudagrass EST SSR primer pair (PP) ID, forward and reverse primer sequences, PCR products and their size for C. dactylon var. aridus 'Giant', C. transvaalensis 'T577', C. dactylon cv. 'Tifton 10', and C. dactylon 'Zebra', SSR repeat motif, expected size and sequence GI number from NCBI
http://www.springerlink.com/content/1513825813877212/
    Last checked: 03/20/2012
    Notes: Abstract only
Keywords:TIC Keywords: Breeding program; Cynodon dactylon; Cynodon transvaalensis; Expressed sequence tag; Gene mapping; Molecular markers; Simple sequence repeats; Sorghum bicolor
Cultivar Names:Tifton 10; Zebra; T577
Abstract/Contents:"Bermudagrass (Cynodon spp.) is extensively cultivated for forage and turf in the the southern United States and in parts of Asia, Africa, southern Europe, Australia and South America. However, few simple sequence repeat (SSR) markers are available for bermudagrass genetics research. Accordingly, the objective of this study was to develop SSR markers in bermudagrass by transferring sorghum genomic SSR primers and by exploring bermudagrass expressed sequence tags (ESTs) from the National Center for Biotechnology Information (NCBI) database. The transferability of 354 tested sorghum SSRs was 57% to C. transvaalensis T577 (2n = 2x = 18), 27% to C. dactylon Tifton 10 (2n = 6x = 54) and 22% to Zebra (2n = 4x = 36). Among the transferred SSRs, 65 primer pairs generated reproducible SSR bands across the three genotypes. From 20,237 Cynodon ESTs at NCBI, 303 designed SSR primer pairs amplified target bands in at least one of C. dactylon var. aridus (2n = 2x = 18), C. transvaalensis T577, C. dactylon cv. Tifton 10, and C. dactylon var. dactylon Zebra. Of the effective EST SSRs, 230 primer pairs produced reproducible bands in all four genotypes. The study demonstrated that EST sequences and sorghum SSR primers are useful sources for the development of SSR markers for bermudagrass. The developed SSR markers will make a valuable contribution to the molecular identification of commercial cultivars, construction of genetic maps, and marker-assisted breeding in bermudagrass."
Language:English
References:38
Note:Figures
Tables
Graphs
ASA/CSSA/SSSA Citation (Crop Science-Like - may be incomplete):
Tan, C., Y. Wu, C. M. Taliaferro, M. P. Anderson, C. Tauer, and T. Samuels. 2012. Development of simple sequence repeat markers for bermudagrass from its expressed sequence tag sequences and preexisting sorghum SSR markers. Molecular Breeding. 29(1):p. 23-30.
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DOI: 10.1007/s11032-010-9521-2
Web URL(s):
https://link.springer.com/article/10.1007%2Fs11032-010-9521-2
    Last checked: 10/05/2017
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